| 存储条件 |
|---|
| Product Name | PP2C alpha [3G1] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Protein phosphatase 1A, Protein phosphatase 2C isoform alpha, Protein phosphatase IA |
| Product description | The phosphorylation and dephosphorylation of proteins on serine and threonine residues is an essential means of regulating a broad range of cellular functions in eukaryotes, including division, homeostasis and apoptosis. A group of proteins that are intimately involved in this process are the serine/ threonine protein phosphatases. Protein phosphatase 2Ca (PP2Ca) has broad specificity. It dephosphorylates and negatively regulates the activities of MAP kinases and MAP kinase-kinases while also inhibiting the activation of p38 and JNK kinase cascades, induced by environmental stresses. PP2Ca also induces the expression of endogenous p53 and the p53-responsive gene p21, leading to cell cycle arrest and apoptosis. The PP2Ca protein, which contains an active site containing a dinuclear metal ion center, shows highest expression in epithelial cells, as well as in the digestive tract, lung, kidney, breast, prostate, endocrine glands and brain. |
| Immunogen | Full length PP2Cα of human origin. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBIP |
| WB:1:100-1:1,000 IP:1-2 μg per 100-500 μg of total protein(1 ml of cell lysate) | |
| Species Reactivity | HumanMouse |
| Concentration | 2mg/ml |
| Alternative Names | Protein phosphatase 1A Protein phosphatase 2C isoform alpha Protein phosphatase IA |
|---|---|
| Molecular Weight(MW) | 46kDa |
| Cellular Localization | Cytoplasm, Nucleus, Membrane |
| SwissProt ID | P35813 |
|---|
Application
Fig1: Western blot analysis of PP2Cα expression in non-transfected 293T (A), human PP2Cα transfected 293T (B) and HeLa (C) whole cell lysates.
Application
Fig2： Western blot analysis of PP2Cα expression in non-transfected 293T (A), human PP2Cα transfected 293T (B) and HeLa (C) whole cell lysates.| Positive Control | Hela, 293T. |
|---|---|
| Application Notes | WB:1:100-1:1,000 IP:1-2 μg per 100-500 μg of total protein(1 ml of cell lysate) |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
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