| 存储条件 |
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| Product Name | Granzyme B [4G9] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | C11 antibody Cathepsin G like 1 antibody Cathepsin G-like 1 antibody CCPI antibody CGL 1 antibody CGL1 antibody CSP B antibody CSPB antibody CTLA-1 antibody CTLA1 antibody CTSGL1 antibody Cytotoxic serine protease B antibody Cytotoxic T lymphocyte associated serine esterase 1 antibody Cytotoxic T lymphocyte proteinase 2 antibody Cytotoxic T-lymphocyte proteinase 2 antibody Fragmentin 2 antibody Fragmentin-2 antibody GRAB_HUMAN antibody Granzyme 2 antibody Granzyme B (granzyme 2, cytotoxic T lymphocyte associated serine esterase 1) antibody Granzyme B antibody Granzyme-2 antibody GranzymeB antibody GRB antibody Gzmb antibody Hlp antibody Human lymphocyte protein antibody Lymphocyte protease antibody Protease, serine, B antibody SECT antibody T cell serine protease 1-3E antibody T-cell serine protease 1-3E antibody |
| Product description | Granzyme A and granzyme B are serine proteases that mediate apoptotic signaling in cytotoxic T lymphocytes (CTL) and natural killer (NK) cells. Both granzyme A and granzyme B are synthesized as inactive proenzymes, and they are stored within cytolytic granules and released by effector cells during degranulation. In activated CTLs, granzyme A and granzyme B are processed and activated by cathepsin C, and they then function to induce apoptosis by two distinct pathways. Granzyme B proteolytically cleaves and activates members of the caspase family of cysteine proteases, including caspase-3, caspase-6, caspase-7 and caspase-9. When cleaved, these caspases assemble into active holoenzymes that then mediate apoptosis through a defined proteolytic cascade involving nuclear lamins and PARP (poly ADP ribose polymerase). Granzyme A mediates the activation of apoptosis by inducing single-strand DNA breaks, membrane perturbation and nuclear condensations in an alternative pathway that is independent from caspase activation or the caspase proteolytic cascade. |
| Immunogen | Amino acids 1-247 representing full length granzyme B of human origin. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBIPIFIHC-P |
| WB:1:100-1:1,000 IP:1-2 μg per 100-500 μg of total protein(1 ml of cell lysate) IF:1:50-500 IHC:1:50-500 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 2mg/ml |
| Alternative Names | C11 antibody Cathepsin G like 1 antibody Cathepsin G-like 1 antibody CCPI antibody CGL 1 antibody CGL1 antibody CSP B antibody CSPB antibody CTLA-1 antibody CTLA1 antibody CTSGL1 antibody Cytotoxic serine protease B antibody Cytotoxic T lymphocyte associated serine esterase 1 antibody Cytotoxic T lymphocyte proteinase 2 antibody Cytotoxic T-lymphocyte proteinase 2 antibody Fragmentin 2 antibody Fragmentin-2 antibody GRAB_HUMAN antibody Granzyme 2 antibody Granzyme B (granzyme 2 cytotoxic T lymphocyte associated serine esterase 1) antibody Granzyme B antibody Granzyme-2 antibody GranzymeB antibody GRB antibody Gzmb antibody Hlp antibody Human lymphocyte protein antibody Lymphocyte protease antibody Protease serine B antibody SECT antibody T cell serine protease 1-3E antibody T-cell serine protease 1-3E antibody |
|---|---|
| Molecular Weight(MW) | 32 kDa |
| Cellular Localization | Cytoplasmic granule |
| SwissProt ID | P10144 |
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Application
Fig1: Western blot analysis of granzyme B expression in non-transfected(A)and human granzyme B transfected(B)293T whole cell lysates.
Application
Fig2： Immunoperoxidase staining of formalin fixed, paraffin-embedded human spleen tissue showing cytoplasmic staining of cells in red pulp.| Positive Control | 293T, human spleen tissue. |
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| Application Notes | WB:1:100-1:1,000 IP:1-2 μg per 100-500 μg of total protein(1 ml of cell lysate) IF:1:50-500 IHC:1:50-500 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
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