| 存储条件 |
|---|
| Product Name | Ku80 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 86 kDa subunit of Ku antigen antibody ATP dependent DNA helicase 2 subunit 2 antibody ATP dependent DNA helicase II 80 kDa subunit antibody ATP dependent DNA helicase II 86 Kd subunit antibody ATP dependent DNA helicase II antibody ATP-dependent DNA helicase 2 subunit 2 antibody ATP-dependent DNA helicase II 80 kDa subunit antibody CTC box binding factor 85 kDa antibody CTC box-binding factor 85 kDa subunit antibody CTC85 antibody CTCBF antibody DNA repair protein XRCC5 antibody KARP 1 antibody KARP1 antibody Ku 80 antibody Ku autoantigen 80kDa antibody Ku80 antibody Ku86 antibody Ku86 autoantigen related protein 1 antibody KUB 2 antibody KUB2 antibody Lupus Ku autoantigen protein p86 antibody NFIV antibody Nuclear factor IV antibody Thyroid lupus autoantigen antibody Thyroid-lupus autoantigen antibody TLAA antibody X ray repair complementing defective repair in Chinese hamster cells 5 (double strand break rejoining) antibody X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining) antibody X-ray repair cross-complementing protein 5 antibody Xray repair complementing defective repair in Chinese hamster cells 5 antibody XRCC 5 antibody XRCC5 antibody XRCC5_HUMAN antibody |
| Product description | Single stranded DNA-dependent ATP-dependent helicase. Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3'-5' direction. Binding to DNA may be mediated by XRCC6. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The XRCC5/6 dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold. The XRCC5/6 dimer is probably involved in stabilizing broken DNA ends and bringing them together. The assembly of the DNA-PK complex to DNA ends is required for the NHEJ ligation step. In association with NAA15, the XRCC5/6 dimer binds to the osteocalcin promoter and activates osteocalcin expression. The XRCC5/6 dimer probably also acts as a 5'-deoxyribose-5-phosphate lyase (5'-dRP lyase), by catalyzing the beta-elimination of the 5' deoxyribose-5-phosphate at an abasic site near double-strand breaks. XRCC5 probably acts as the catalytic subunit of 5'-dRP activity, and allows to 'clean' the termini of abasic sites, a class of nucleotide damage commonly associated with strand breaks, before such broken ends can be joined. The XRCC5/6 dimer together with APEX1 acts as a negative regulator of transcription. |
| Immunogen | Peptide |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBICCIHC |
| WB:1:500~1:2000 ICC:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Alternative Names | 86 kDa subunit of Ku antigen antibody ATP dependent DNA helicase 2 subunit 2 antibody ATP dependent DNA helicase II 80 kDa subunit antibody ATP dependent DNA helicase II 86 Kd subunit antibody ATP dependent DNA helicase II antibody ATP-dependent DNA helicase 2 subunit 2 antibody ATP-dependent DNA helicase II 80 kDa subunit antibody CTC box binding factor 85 kDa antibody CTC box-binding factor 85 kDa subunit antibody CTC85 antibody CTCBF antibody DNA repair protein XRCC5 antibody KARP 1 antibody KARP1 antibody Ku 80 antibody Ku autoantigen 80kDa antibody Ku80 antibody Ku86 antibody Ku86 autoantigen related protein 1 antibody KUB 2 antibody KUB2 antibody Lupus Ku autoantigen protein p86 antibody NFIV antibody Nuclear factor IV antibody Thyroid lupus autoantigen antibody Thyroid-lupus autoantigen antibody TLAA antibody X ray repair complementing defective repair in Chinese hamster cells 5 (double strand break rejoining) antibody X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining) antibody X-ray repair cross-complementing protein 5 antibody Xray repair complementing defective repair in Chinese hamster cells 5 antibody XRCC 5 antibody XRCC5 antibody XRCC5_HUMAN antibody |
|---|---|
| Molecular Weight(MW) | 83 kD |
| Cellular Localization | Nucleus. Chromosome. |
| SwissProt ID | P13010 |
|---|
Application
Fig1: Western blot analysis of Ku80 on different cell lysate using anti-Ku80 antibody at 1/500 dilution. Positive control： Lane1: A549 Lane2: Hela Lane3: MCF-7 Lane4: Human liver
Application
Fig2: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Ku80 antibody. Counter stained with hematoxylin.
Application
Fig3: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Ku80 antibody. Counter stained with hematoxylin.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-Ku80 antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Ku80 antibody. Counter stained with hematoxylin.| Positive Control | A549, Hela, MCF-7, human liver tissue lysate, human tonsil tissue, human colon cancer tissue, human breast cancer tissue, human kidney tissue. |
|---|---|
| Application Notes | WB:1:500~1:2000 ICC:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
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