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Anti-SDF1 antibody

产品编号 : ab9797

英文名称 :

中文名称 : 抗体

产品等级 :

品牌 : Abcam

包装规格	交货周期	质量标准	目录价	会员专享价	数量
100ug			￥6177	登录后可见	- +

产品详情

IHC-P: Human lung cancer and human kidney tissues; WB: Recombinant human SDF1 protein (ab9798).

常规说明
Although some customers have been successful in IHC we no longer batch test in this application.

The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
形式
Lyophilized:Reconstitute with 200µl of sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
存储溶液
Constituent: PBS

No preservative, sterile filtered
纯度
Immunogen affinity purified
克隆
多克隆
同种型
unknown
轻链类型
unknown

Abpromise™承诺保证使用ab9797于以下的经测试应用

“应用说明”部分下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用	Ab评论	说明
Sandwich ELISA		Use a concentration of 0.5 - 2 µg/ml. Can be paired for Sandwich ELISA with Biotin Rabbit polyclonal to SDF1 (ab84277). (using 100 µl/well antibody solution). This antigen affinity purified antibody, in conjunction with ab84277 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human SDF1 alpha. Read More
WB		Use at an assay dependent concentration. To detect hSDF-1a by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hSDF-1a is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. Read More
IHC-P	(8)	Use a concentration of 2 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SDF1 antibody (ab9797)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling SDF1 with ab9797 at 2 µg/mL (45 minute incubation at room temperature). Heat mediated antigen retrieval was performed using a buffer at pH 6. An HRP-labeled polymer detection system was used with a DAB chromogen.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SDF1 antibody (ab9797)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung cancer tissue labelling SDF1 with ab9797 at 2 µg/mL (45 minute incubation at room temperature). Heat mediated antigen retrieval was performed using a buffer at pH 6. An HRP-labeled polymer detection system was used with a DAB chromogen.
Sandwich ELISA - Anti-SDF1 antibody (ab9797)
ELISA - Anti-IL-1 beta antibody (ab9797)
Sandwich ELISA detecting SDF1 using ab9797 at a concentration of 2.0 µg/ml.
Western blot - Anti-SDF1 antibody (ab9797)
To detect human SDSF1 by Western Blot analysis, ab9739 can be used at a concentration of 0.1-0.2 μg/ml. When used in conjunction with compatible development reagents, the detection limit for recombinant human SDF1 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Lanes 1-11: 250, 125, 62.5, 31.25, 15.625, 7.8, 3.9, 1.95, 0.975, 0.4875 and 0.24 ng recombinant human SDF1, respectively.
Non-reducing conditions.