Anti-TNF alpha antibody [NF-7]

• 产品名称

  Anti-TNF alpha抗体[NF-7]
  参阅全部 TNF alpha 一抗

• 描述

  小鼠单克隆抗体[NF-7] to TNF alpha

• 宿主

  Mouse

• 经测试应用

  适用于: Flow Cytmore details

• 种属反应性

  与反应: Human
  

• 免疫原

  Recombinant full length protein corresponding to Human TNF alpha.
  Database link: P01375

• 常规说明

  This antibody can be used in two-site immunoassay for the determination of human TNF alpha. It recognizes a different epitope than the monoclonal antibody ab9813. The two-site immunoassay can be used to determine TNF alpha concentration in the cultures of human peripheral blood mononuclear cells. Can also be used to neutralize TNF alpha cytotoxic activity in vitro. At a concentration of 2.5 µg/ml, antibody ab9809 causes 50% inhibition of TNF alpha mediated cytotoxicity on L929 cells.

  This product was changed from ascites to tissue culture supernatant on 28/11/2017. Lot numbers higher than GR151594-8 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.

  This product was changed from ascites to tissue culture supernatant on 28/11/2017. Lot numbers higher than GR151594-8 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.

  This antibody can be used in two-site immunoassay for the determination of human TNF alpha. It recognizes a different epitope than the monoclonal antibody ab9813. The two-site immunoassay can be used to determine TNF alpha concentration in the cultures of human peripheral blood mononuclear cells. Can also be used to neutralize TNF alpha cytotoxic activity in vitro. At a concentration of 2.5 µg/ml, antibody ab9809 causes 50% inhibition of TNF alpha mediated cytotoxicity on L929 cells.

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  pH: 7.2
  Preservative: 0.1% Sodium azide

• 纯度

  Protein A purified

• Primary antibody说明

  This antibody can be used in two-site immunoassay for the determination of human TNF alpha. It recognizes a different epitope than the monoclonal antibody ab9813. The two-site immunoassay can be used to determine TNF alpha concentration in the cultures of human peripheral blood mononuclear cells. Can also be used to neutralize TNF alpha cytotoxic activity in vitro. At a concentration of 2.5 µg/ml, antibody ab9809 causes 50% inhibition of TNF alpha mediated cytotoxicity on L929 cells.

• 克隆

  单克隆

• 克隆编号

  NF-7

• 骨髓瘤

  NS0/1

• 同种型

  IgG1

• 轻链类型

  unknown

The Abpromise guarantee

Abpromise™承诺保证使用ab9809于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 7124 Human

• Omim: 191160 Human

• SwissProt: P01375 Human

• Unigene: 241570 Human

• 别名

• DIF antibody

• Differentiation inducing factor antibody

• Macrophage cytotoxic factor antibody

• Tnf antibody

• TNF superfamily member 2 antibody

• TNF superfamily, member 2 antibody

• TNF, macrophage derived antibody

• TNF, monocyte derived antibody

• TNF-a antibody

• TNF-alpha antibody

• TNFA antibody

• TNFA_HUMAN antibody

• TNFSF2 antibody

• Tumor necrosis factor (TNF superfamily member 2) antibody

• Tumor necrosis factor alpha antibody

• Tumor necrosis factor antibody

• Tumor necrosis factor ligand superfamily member 2 antibody

• Tumor Necrosis Factor, Membrane Form antibody

• Tumor necrosis factor, soluble form antibody

• APC1 antibody

• APC1 protein antibody

• Cachectin antibody

• 

  Flow Cytometry - Anti-TNF alpha antibody [NF-7] (ab9809)

  Overlay histogram showing THP1 cells stained with ab9809 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab9809, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.