Anti-M-CSF抗体
参阅全部 M-CSF 一抗
兔多克隆抗体to M-CSF
Rabbit
适用于: WB, IHC-P, ELISA, Neutralising, Sandwich ELISAmore details
与反应: Human, Recombinant fragment
Recombinant full length protein corresponding to Human M-CSF. Highly pure (>98%) recombinant hM-CSF (human M-CSF)
Database link: P09603
WB: Recombinant human M-CSF protein (ab9694).
This product is no longer batch tested in IHC, for an IHC validated antibody please see ab52864
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Lyophilized:Reconstitute with 200µl of sterile water.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
No preservative, sterile filtered
Immunogen affinity purified
多克隆
unknown
unknown
Abpromise™承诺保证使用ab9693于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (1) | Use at an assay dependent concentration. To detect hM-CSF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hM-CSF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. Read More |
| IHC-P | Use at an assay dependent concentration. | |
| ELISA | Use at an assay dependent concentration. To detect hM-CSF by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hM-CSF. Read More | |
| Neutralising | Use at an assay dependent concentration. To yield one-half maximal inhibition [ND50] of the biological activity of hM-CSF (7.0 ng/ml), a concentration of 0.02 - 0.03 µg/ml of this antibody is required. | |
| Sandwich ELISA | Use a concentration of 0.1 µg/ml. To detect hM-CSF by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. |
Entrez Gene: 1435 Human
Omim: 120420 Human
SwissProt: P09603 Human
Unigene: 591402 Human
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Western blot - Anti-M-CSF antibody (ab9693)
Anti-M-CSF antibody (ab9693) at 0.2 µg/ml + Recombinant human M-CSF protein (ab9694) at 0.01 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 1 minute
Western blot - Anti-M-CSF antibody (ab9693)This image is courtesy of an Abreview submitted by Valentina Marcelli
All lanes : Anti-M-CSF antibody (ab9693) at 1/500 dilution (for 12 hours at 4°C)
Lane 1 : Human Kidney whole cell lysate
Lane 2 : Human Kidney whole cell lysate transfected with LFABP
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : An IRDye®800CW-conjugated Goat anti-rabbit IgG polyclonal at 1/15000 dilution
Performed under non-reducing conditions.
Observed band size: 70 kDawhy is the actual band size different from the predicted?
Blocking Step: 5% BSA for 1 hour at 25°C
Deatection method: Odyssey for 50 seconds.
Right-hand Image: Weaker exposure to identify bands.
Sandwich ELISA - Anti-M-CSF antibody (ab9693)
Standard curve for MCSF (Analyte: ab62015); dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal [21] to MCSF (ab18645) at 1µg/ml and Detector Antibody Rabbit polyclonal to MCSF (ab9693) at 0.1µg/ml.
