Anti-IL-1 beta抗体
参阅全部 IL-1 beta 一抗
兔多克隆抗体to IL-1 beta
Rabbit
Anti-IL-1 beta antibody is expected to react with both the mature and pro form of IL-1 beta.
适用于: IHC-P, Sandwich ELISA, WBmore details
与反应: Human, Recombinant fragment
Full length protein aa 118-269. Full length mature protein minus the propeptide from aa 1-117 (Peptide available as ab9723).
Database link: P10749
IHC-P: Human adrenal tissue; Recombinant mouse IL-1 beta protein (ab9723) can be used as a positive control in WB
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Lyophilized:Reconstitute with sterile water to 0.1-1.0mg/ml, aliquot and store at -20°C.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle.
No preservative, sterile filtered
Immunogen affinity purified
多克隆
unknown
unknown
Abpromise™承诺保证使用ab9722于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | (5) | Use a concentration of 2 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
| Sandwich ELISA | Use a concentration of 0.5 - 2 µg/ml. To detect mIL-1b by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant mIL-1b. Read More | |
| WB | (6) | Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 30 kDa. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-1β is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. |
Entrez Gene: 3553 Human
Omim: 147720 Human
SwissProt: P01584 Human
Unigene: 126256 Human
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Preinterleukin 1 beta antibody
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Pro interleukin 1 beta antibody
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IFN beta inducing factor antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-1 beta antibody (ab9722)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human normal adrenal tissue labelling IL-1 beta with ab9722 at 2 µg/mL (45 minute incubation at room temperature). Heat mediated antigen retrieval was performed using a buffer at pH 6. An HRP-labeled polymer detection system was used with a DAB chromogen.
Sandwich ELISA - Anti-IL-1 beta antibody (ab9722)
Sandwich ELISA detecting IL-1 beta using ab9722 at a concentration of 2 µg/ml.
Western blot - Anti-IL-1 beta antibody (ab9722)
To detect mouse IL-1 beta by Western Blot analysis, ab9722 can be used at a concentration of 0.1-0.2 μg/ml. When used in conjunction with compatible development reagents, the detection limit for recombinant mouse IL-1 beta is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Lanes 1-11: 250, 125, 62.5, 31.25, 15.625, 7.8, 3.9, 1.95, 0.975, 0.4875 and 0.24 ng recombinant mouse IL-1 beta, respectively.
Non-reducing conditions.
