Anti-PUMA抗体
参阅全部 PUMA 一抗
兔多克隆抗体to PUMA
Rabbit
At least 2 isoforms are known to exist; this antibody will detect both isoforms.
适用于: WB, ICC/IFmore details
与反应: Mouse, Human
Synthetic peptide corresponding to Human PUMA aa 150-250 (C terminal).
Apoptosis is related to many diseases and development. The p53 tumor-suppressor protein induces apoptosis through transcriptional activation of several genes. A novel p53 inducible pro-apoptotic gene was identified recently and designated PUMA (for p53 upregulated modulator of apoptosis) and bbc3 (for Bcl-2 binding component 3) in human and mouse (1-3). PUMA/bbc3 is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53. The PUMA gene encodes two BH3 domain-containing proteins termed PUMA-a and PUMA-b (1). PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis.
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Liquid
Shipped at 4°C. Store at +4°C.
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS
Immunogen affinity purified
Apoptosis is related to many diseases and development. The p53 tumor-suppressor protein induces apoptosis through transcriptional activation of several genes. A novel p53 inducible pro-apoptotic gene was identified recently and designated PUMA (for p53 upregulated modulator of apoptosis) and bbc3 (for Bcl-2 binding component 3) in human and mouse (1-3). PUMA/bbc3 is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53. The PUMA gene encodes two BH3 domain-containing proteins termed PUMA-a and PUMA-b (1). PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis.
多克隆
IgG
Abpromise™承诺保证使用ab9643于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (9) | Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 23 kDa. Use at a concentration of 1 - 2 µg/ml. Detects a band of approximately 23 kDa. Can be blocked with PUMA peptide (180/193) (ab9644). A lower band at approximately 16 kDa was detected in MOLT4 and U937 cells, which may represent the PUMA-beta form. Read More |
| ICC/IF | Use a concentration of 1 µg/ml. |
Entrez Gene: 27113 Human
Entrez Gene: 170770 Mouse
Omim: 605854 Human
SwissProt: Q9BXH1 Human
SwissProt: Q99ML1 Mouse
Unigene: 467020 Human
Unigene: 7660 Mouse
BCL 2 binding component 3 antibody
Bcl-2-binding component 3 antibody
BCL2 binding component 3 antibody
JFY 1 antibody
JFY-1 antibody
JFY1 antibody
p53 up regulated modulator of apoptosis antibody
p53 up-regulated modulator of apoptosis antibody
p53 Upregulated Modulator of Apoptosis antibody
PUMA alpha antibody
PUMA/JFY1 antibody
BBC 3 antibody
Bbc3 antibody
BBC3_HUMAN antibody
Western blot - Anti-PUMA antibody (ab9643)
All lanes : Anti-PUMA antibody (ab9643) at 2 µg/ml
Lane 1 : HEK293 cells were transfected with control siRNAs
Lane 2 : HEK293 cells were transfected with PUMA siRNAs
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution
Beta-actin (1 μg/mL) and GAPDH (0.02 μg/mL). Incubation time: 1 hour at Room Temperature in 5% NFDM/TBST.
Immunocytochemistry/ Immunofluorescence - Anti-PUMA antibody (ab9643)
Immunofluorescent analysis of 4% paraformaldehydefixed K562 cells labeling PUMA with ab9643 at 2 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red). Image showing cytosol staining on K562 cells.
Immunocytochemistry/ Immunofluorescence - Anti-PUMA antibody (ab9643)
ICC/IF image of ab9643 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab9643, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Immunocytochemistry/ Immunofluorescence - Anti-PUMA antibody (ab9643)
Immunocytochemical analysis of K562 cells labeling PUMA with ab9643 at 1 μg/mL. Cells were fixed with formaldehyde and blocked with 10% serum for 1 hour at room temperature. Antigen retrieval was by heat mediation with a citrate buffer (pH 6.0). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Western blot - Anti-PUMA antibody (ab9643)
All lanes : Anti-PUMA antibody (ab9643) at 2 µg/ml
Lane 1 : K562 cell lysate
Lane 2 : NIH3T3 cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution
Western blot - Anti-PUMA antibody (ab9643)This image is courtesy of an anonymous Abreview
Anti-PUMA antibody (ab9643) at 1/1000 dilution + HeLa whole cell lysate
Secondary
Alexa Fluor 680-conjugated goat anti-rabbit IgG polyclonal at 1/1 dilution
Observed band size: 20 kDawhy is the actual band size different from the predicted?
Additional bands at: 26 kDa (possible non-specific binding), 42 kDa (possible non-specific binding), 50 kDa (possible non-specific binding)
Exposure time: 5 seconds
Blocked with 5% milk for 1 hour.
Incubated with the primary antibody for 18 hours.
