Anti-FLASH抗体
兔多克隆抗体to FLASH
Rabbit
适用于: WB, ICC/IFmore details
与反应: Human
预测可用于: Mouse
Synthetic peptide corresponding to Human FLASH aa 1950-2050.
HeLa cell lysate An approximate 220 kDa band can be detected.
A novel mammalian CED-4 homologous was recently identified and cloned in mouse (1) and human (2) and designated FLASH (for FLICE-associated huge protein). FLASH is involved in Fas induced apoptosis. It is recruited to Fas after the receptor cross-linking. Overexpression of wild type of FLASH facilitates and its dominant negative form inhibits Fas induced apoptosis. FLASH interacts with the DEDs of caspase-8 and FADD through the DED-like domain of FLASH and mediates activation of caspase-8 (1). There are parallels between FLASH and Apaf-1/CED-4 although there are arguments against their structural similarity (1,2).
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
pH: 7.2
Preservative: 0.02% Sodium azide
Affinity purified
A novel mammalian CED-4 homologous was recently identified and cloned in mouse (1) and human (2) and designated FLASH (for FLICE-associated huge protein). FLASH is involved in Fas induced apoptosis. It is recruited to Fas after the receptor cross-linking. Overexpression of wild type of FLASH facilitates and its dominant negative form inhibits Fas induced apoptosis. FLASH interacts with the DEDs of caspase-8 and FADD through the DED-like domain of FLASH and mediates activation of caspase-8 (1). There are parallels between FLASH and Apaf-1/CED-4 although there are arguments against their structural similarity (1,2).
多克隆
IgG
unknown
Abpromise™承诺保证使用ab8420于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (1) | Use a concentration of 0.25 - 0.5 µg/ml. Detects a band of approximately 220 kDa. |
| ICC/IF | Use a concentration of 10 µg/ml. |
Entrez Gene: 9994 Human
Entrez Gene: 26885 Mouse
Omim: 606880 Human
SwissProt: Q9UKL3 Human
SwissProt: Q9WUF3 Mouse
Unigene: 558218 Human
Unigene: 22279 Mouse
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Western blot - Anti-FLASH antibody (ab8420)
Anti-FLASH antibody (ab8420) at 0.5 µg/ml + HeLa whole cell lysate
Observed band size: 220 kDawhy is the actual band size different from the predicted?
Immunocytochemistry/ Immunofluorescence - Anti-FLASH antibody (ab8420)
ab8420 at 10µg/ml staining FLASH in Hela cells by ICC/IF
