Anti-IL-4抗体
参阅全部 IL-4 一抗
兔多克隆抗体to IL-4
Rabbit
适用于: ELISA, WB, Sandwich ELISA, Neutralisingmore details
与反应: Human
Recombinant full length protein corresponding to Human IL-4. Highly pure (>98%) recombinant hIL-4 (human Interleukin-4)
Database link: P05112
Recombinant human IL4 protein (ab9623) can be used as a positive control in WB.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Lyophilized:Reconstitute with 200µl of sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
No preservative, sterile filtered
Immunogen affinity purified
多克隆
IgG
Abpromise™承诺保证使用ab9622于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ELISA | Use at an assay dependent concentration. To detect hIL-4 by direct ELISA (using 100 µl/well antibody solution) a concentration of at least 0.5 µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection. Read More | |
| WB | Use at an assay dependent concentration. Detects a band of approximately 15 kDa. To detect hIL-4 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-4 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. Detects a band of approximately 15 kDa. Read More | |
| Sandwich ELISA | Use at an assay dependent concentration. Can be used as Detection antibody with recommended pair. | |
| Neutralising | Use at an assay dependent concentration. To yield one-half maximal inhibition [ND50] of the biological activity of hIL-4 (1.50 ng/ml), a concentration of 0.03 - 0.05 µg/ml of this antibody is required. |
Entrez Gene: 3565 Human
Omim: 147780 Human
SwissProt: P05112 Human
Unigene: 73917 Human
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Western blot - Anti-IL-4 antibody (ab9622)
