Anti-Phosphothreonine-Proline / Phosphoserine-Proline抗体
兔多克隆抗体to Phosphothreonine-Proline / Phosphoserine-Proline
Rabbit
This antibody specifically reacts to proteins containing phosphothreonine-proline motifs, it also reacts to the phosphoserine-proline motif to a similar degree (pT-P and pS-P motif). The antibody does not react to phosphothreonine, phosphoserine or phosphotyrosine. The antibody will selectively recognize the phosphothreonine-proline motif of MBP, which is the site of phosphorylation by ERK1/ERK2.
适用于: ELISA, WBmore details
与反应: Species independent
Chemical/ Small Molecule corresponding to Phosphothreonine-Proline/ Phosphoserine-Proline conjugated to keyhole limpet haemocyanin.
WB: Phosphorylated MBP
The antigen is based on N-acetyl-pT-K-pY-NH2. The pT-X-pY sequence is considered to be the motif for some active MAPKs.
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 6
Preservative: 0.02% Sodium azide
Immunogen affinity purified
Immunoaffinity chromatography with phosphothreonine-proline on agarose, then immuno-absorption with threonine-proline-NH2 on agarose.
The antigen is based on N-acetyl-pT-K-pY-NH2. The pT-X-pY sequence is considered to be the motif for some active MAPKs.
多克隆
IgG
Abpromise™承诺保证使用ab9344于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ELISA | Use a concentration of 0.5 µg/ml. | |
| WB | Use a concentration of 2 µg/ml. |
Western blot - Anti-Phosphothreonine-Proline / Phosphoserine-Proline antibody (ab9344)
All lanes : Anti-Phosphothreonine-Proline / Phosphoserine-Proline antibody (ab9344)
Lane 1 : Phosphorprotein protein kinase A PKA
Lane 2 : Myelin Basic Protein (MBP)
