Anti-IGF1+IGF2抗体
兔多克隆抗体to IGF1 + IGF2
Rabbit
适用于: ICC/IF, IHC-FoFr, WB, Neutralising, IHC-P, Sandwich ELISAmore details
与反应: Mouse, Human, Recombinant fragment
Recombinant full length protein corresponding to Human IGF1. Highly pure (>98%) recombinant hIGF-1 (human Insulin Like Growth Factor-1).
Database link: P05019
IHC-P: Human pancreas and liver tissues.
Although some customers have been successful in IHC we no longer batch test in this application. For an IHC validated antibody please see ab263903
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Lyophilized:Reconstitute with 200µl of sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Immunogen affinity purified
多克隆
IgG
unknown
Abpromise™承诺保证使用ab9572于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | (2) | Use at an assay dependent concentration. |
| IHC-FoFr | (1) | Use at an assay dependent concentration. PubMed: 28754163 |
| WB | (2) | Use at an assay dependent concentration. Predicted molecular weight: 22 kDa. To detect hIGF-1 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIGF-1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. Read More |
| Neutralising | Use at an assay dependent concentration. To yield one-half maximal inhibition of the biological activity of hIGF-1 (5.0 ng/ml), a concentration of 0.67 - 1.0 ug/ml of this antibody is required. | |
| IHC-P | (1) | Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
| Sandwich ELISA | Use a concentration of 0.5 - 2 µg/ml. Can be paired for Sandwich ELISA with Biotin Rabbit polyclonal to IGF1 (ab83137). Allows for the detection of at least 0.2 - 0.4 ng/well of recombinant IGF1. |
IGF1: Secreted. IGF2: Secreted.
SwissProt: P05019 Human
SwissProt: P05017 Mouse
SwissProt: P09535 Mouse
Unigene: 160562 Human
Unigene: 272259 Human
Unigene: 718427 Human
Unigene: 268521 Mouse
Unigene: 3862 Mouse
Entrez Gene: 3479 Human
Entrez Gene: 3481 Human
Entrez Gene: 16000 Mouse
Entrez Gene: 16002 Mouse
Omim: 147440 Human
Omim: 147470 Human
SwissProt: P01343 Human
SwissProt: P01344 Human
Western blot - Anti-IGF1+IGF2 antibody (ab9572)
All lanes : Anti-IGF1+IGF2 antibody (ab9572) at 1/1000 dilution
Lanes 1 & 3 : Mouse IGF-2 recombinant protein (aa25-91, without tag)
Lanes 2 & 4 : Mouse IGF-1 recombinant protein (aa25-91, without tag)
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 22 kDa
Exposure time: 59 seconds
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGF1+IGF2 antibody (ab9572)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human pancreas tissue labelling IGF1 with ab9572 at 1 µg/mL (45 minute incubation at room temperature). Heat mediated antigen retrieval was performed using a buffer at pH 6. An HRP-labeled polymer detection system was used with a DAB chromogen.
Sandwich ELISA - Anti-IGF1+IGF2 antibody (ab9572)
To detect hIGF-I by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human IGF-I as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIGF-I.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGF1+IGF2 antibody (ab9572)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue labelling IGF1 with ab9572 at 1 µg/mL (45 minute incubation at room temperature). Heat mediated antigen retrieval was performed using a buffer at pH 6. An HRP-labeled polymer detection system was used with a DAB chromogen.
