Recombinant human VEGF 165A protein (Active)

• 产品名称

  重组人VEGF 165A蛋白(Active)
  参阅全部 VEGF 165A 蛋白酶

• 生物活性

  Determined by its ability to stimulate the proliferation of human umbilical vein endothelial cells (HUVEC). The expected ED₅₀ for this effect is 1.0-10.0ng/ml.

• 纯度

  > 98 % SDS-PAGE.
  > 98% HPLC analyses. Sterile filtered
  

• 内毒素水平

  < 1.000 Eu/µg

• 表达系统

  Escherichia coli

• Accession

  P15692-4

• 蛋白长度

  Full length protein

• 无动物成分

  No

• 性质

  Recombinant

• 
  

• 种属

  Human

• 序列

  APMAEGGGQN HHEVVKFMDV YQRSYCHPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCGGC CNDEGLECVP TEESNITMQI MRIKPHQGQH IGEMSFLQHN KCECRPKKDR ARQENPCGPC SERRKHLFVQ DPQTCKCSCK NTDSRCKARQ LELNERTCRC DKPRR

• 预测分子量

  38 kDa

• 氨基酸

  27 to 191

• 额外的序列信息

  aa 27-232 is the full length mature protein minus the signal peptide.

Our Abpromise guarantee covers the use of ab9571 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

• 应用

  HPLC

  Cell Differentiation

  Cellular Activation

  Functional Studies

  SDS-PAGE

• 形式

  Lyophilized

• 稳定性和存储

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  This product is an active protein and may elicit a biological response in vivo, handle with caution.

• 复溶

  Reconstitute in 100 µl dH2O

别名

• glioma-derived endothelial cell mitogen

• MGC70609

• MVCD1

• Vascular Endothelial Growth Factor

• Vascular endothelial growth factor A

• Vascular permeability factor

• VEGF

• VEGF-A

• VEGF165

• Vegfa

• VEGFA_HUMAN

• VPF

• 

  Cell differentiation - Recombinant human VEGF 165A protein (Active) (ab9571)This image is courtesy of Sun Yung, Centro de Investigación Príncipe Felipe.

  Human embryonic stem cells (H9) were differentiated both as embryoid bodies and monolayer cultures (on Matrigel) using a protocol described in Kennedy et al., 2006. BMP-4, FGF-basic (ab9596) and VEGF (ab9571) were sequentially added to the media to direct differentiation. These images were taken at day 8, where cell adhesion antigens CD31 (ab32457 in green) and CD34 (ab8536 in red) are abundant and represent primitive haematopoietic cells within the culture. DAPI staining is shown in blue.