Anti-Aquaporin 1 antibody [1/22]

• 产品名称

  Anti-Aquaporin 1抗体[1/22]
  参阅全部 Aquaporin 1 一抗

• 描述

  小鼠单克隆抗体[1/22] to Aquaporin 1

• 宿主

  Mouse

• 经测试应用

  适用于: WB, IHC-P, Flow Cytmore details

• 种属反应性

  与反应: Rat, Human
  预测可用于: Sheep, Cow

• 免疫原

  Synthetic peptide:

  GQVEEYDLDDDINSRVEMKPK

  , corresponding to amino acids 249-269 of Aquaporin 1.

  Run BLAST with Run BLAST with 

• 表位

  Intracellular C-terminal AQP 1 epitope (active channel loop)

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  Preservative: 0.097% Sodium azide
  Constituents: PBS, 0.2% BSA

• 浓度

• 100 µg 浓度为 1 mg/ml

• 纯度

  Protein A purified

• 克隆

  单克隆

• 克隆编号

  1/22

• 同种型

  IgG2b

• 轻链类型

  kappa

The Abpromise guarantee

Abpromise™承诺保证使用ab9566于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 358 Human

• Entrez Gene: 25240 Rat

• Omim: 107776 Human

• SwissProt: P29972 Human

• SwissProt: P29975 Rat

• Unigene: 76152 Human

• Unigene: 1618 Rat

• 别名

• AQP1 antibody

• AQP1_HUMAN antibody

• aquaporin 1 (channel-forming integral protein, 28kDa, CO blood group) antibody

• aquaporin 1 (Colton blood group) antibody

• Aquaporin CHIP antibody

• Aquaporin-1 antibody

• Aquaporin-CHIP antibody

• Aquaporin1 antibody

• Channel forming integral protein 28kDa antibody

• Channel like integral membrane protein 28 kDa antibody

• CHIP 28 antibody

• CHIP28 antibody

• CO antibody

• Colton blood group antibody

• Growth factor induced delayed early response protein antibody

• MGC26324 antibody

• Urine water channel antibody

• Water channel protein CHIP 29 antibody

• Water channel protein CHIP29 antibody

• Water channel protein for red blood cells and kidney proximal tubule antibody

• AQP 1 antibody

• AQP CHIP antibody

• AQP-1 antibody

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 1 antibody [1/22] (ab9566)

  Immunohistochemical staining of aquaporin 1 in rat brain.

• 

  Flow Cytometry - Anti-Aquaporin 1 antibody [1/22] (ab9566)

  Overlay histogram showing HEK293 cells stained with ab9566 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Triton for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab9566, 2µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HEK293 cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Triton used under the same conditions.
  
  

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 1 antibody [1/22] (ab9566)

  Immunohistochemical analysis of exocrine pancreas cells labeling Aquaporin 1 with ab9566. 

• 

  Western blot - Anti-Aquaporin 1 antibody [1/22] (ab9566)

  Anti-Aquaporin 1 antibody [1/22] (ab9566) at 1 µg/ml + Human skeletal muscle tissue lysate - total protein (ab29330) at 10 µg
  
  Secondary
  Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
  
  Developed using the ECL technique.
  
  Performed under reducing conditions.
  
  Predicted band size: 29 kDa
  Observed band size: 29 kDa
  Additional bands at: 50 kDa, 75 kDa. We are unsure as to the identity of these extra bands.
  
  
  Exposure time: 30 seconds