Anti-Integrin beta 3抗体[Y2/51]
参阅全部 Integrin beta 3 一抗
小鼠单克隆抗体[Y2/51] to Integrin beta 3
Mouse
适用于: Flow Cytmore details
不适用于: ICC/IF,IHC-P or WB
与反应: Human
Tissue, cells or virus corresponding to Human Integrin beta 3. PHA-stimulated human mononuclear cells.
Flow cyto: Human peripheral blood mononuclear cell (PBMC) cells
This product has been switch from hybridoma to recombinant on 25th March 2024.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information see here.
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free production
Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), 59% PBS
浓度
250 µl 浓度为 1.12 mg/ml
Protein A purified
单克隆
Y2/51
unknown
IgG1
kappa
Abpromise™承诺保证使用ab9509于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
Flow Cyt | 1/1000. |
应用说明
Is unsuitable for ICC/IF,IHC-P or WB.
Entrez Gene: 3690 Human
Omim: 173470 Human
SwissProt: P05106 Human
Unigene: 218040 Human
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Flow Cytometry - Anti-Integrin beta 3 antibody [Y2/51] (ab9509)
Flow cytometric analysis of Human peripheral blood mononuclear cell (PBMC) cells labelling Integrin beta 3 with ab9509 at 1/1000 dilution (0.1 ug) / Right compared with a Mouse monoclonal IgG / Left.
Goat anti mouse IgG (Alexa Fluor® 488, ab150113) was used as the secondary antibody at 1/2000 dilution.
Cells were stained with mouse IgG or ab9509. Then stained with anti-CD41 conjugated to APC.
Gated on viable cells.
Flow Cytometry - Anti-Integrin beta 3 antibody [Y2/51] (ab9509)
Flow cytometric analysis of HeLa (Human cervix adenocarcinoma epithelial cell, Left) / HUVEC (Human umbilical vein endothelial cell, Right) cells labelling Integrin beta 3 with ab9509 at 1/1000 dilution (0.1 ug)/Red compared with a Mouse monoclonal IgG / Black isotype control and an unlabelled control (Cell without incubation with primary antibody and secondary antibody / Blue).
Secondary antibody was Goat anti mouse IgG (Alexa Fluor® 488, ab150113) used at 1/2000 dilution.
Negative control: HeLa.
Gated on viable cells.
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