Anti-EBP50/NHERF-1 antibody [EBP-10]

• 产品名称

  Anti-EBP50/NHERF-1抗体[EBP-10]
  参阅全部 EBP50/NHERF-1 一抗

• 描述

  小鼠单克隆抗体[EBP-10] to EBP50/NHERF-1

• 宿主

  Mouse

• 特异性

  Human ERM (ezrin/radixin/moesin)-binding phosphoprotein of 50 kDa (EBP50) / Na+/H+ exchange regulatory factor (NHERF-1)

• 经测试应用

  适用于: Flow Cyt, WBmore details

• 种属反应性

  与反应: Human
  

• 免疫原

  Recombinant full length protein corresponding to Human EBP50/NHERF-1.

• 阳性对照

• RAJI human lymphoma cell lysate.

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  pH: 7.40
  Preservative: 0.097% Sodium azide
  Constituent: PBS

• 浓度

• 100 µg 浓度为 1 mg/ml

• 纯度

  Protein A purified

• 纯化说明

  Purified from hybridoma culture supernatant. Purity >95% by SDS-PAGE.

• 克隆

  单克隆

• 克隆编号

  EBP-10

• 同种型

  IgG2b

The Abpromise guarantee

Abpromise™承诺保证使用ab9526于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 9368 Human

• Omim: 604990 Human

• SwissProt: O14745 Human

• Unigene: 724482 Human

• Unigene: 728760 Human

• 别名

• Ezrin-radixin-moesin-binding phosphoprotein 50 antibody

• Na(+)/H(+) exchange regulatory cofactor NHE RF antibody

• Na(+)/H(+) exchange regulatory cofactor NHE-RF1 antibody

• Na+/H+ exchange regulatory co factor antibody

• NHERF 1 antibody

• NHERF antibody

• NHERF-1 antibody

• NHERF1 antibody

• NHRF1_HUMAN antibody

• NPHLOP2 antibody

• Regulatory cofactor of Na(+)/H(+) exchanger antibody

• Slc9a3r1 antibody

• Sodium hydrogen exchanger regulatory factor 1 antibody

• Sodium-hydrogen exchanger regulatory factor 1 antibody

• Sodium/hydrogen exchanger regulatory factor 1 antibody

• Solute carrier family 9 (sodium/hydrogen exchanger) member 3 regulator 1 antibody

• Solute carrier family 9 (sodium/hydrogen exchanger), isoform 3 regulatory factor 1 antibody

• Solute carrier family 9 isoform 3 regulatory factor 1 antibody

• Solute carrier family 9 isoform A3 regulatory factor 1 antibody

• Solute carrier family 9 member 3 regulator 1 antibody

• EBP 50 antibody

• EBP50 antibody

• Ezrin radixin moesin binding phosphoprotein 50 antibody

• 

  Western blot - Anti-EBP50/NHERF-1 antibody [EBP-10] (ab9526)

  All lanes : Anti-EBP50/NHERF-1 antibody [EBP-10] (ab9526) at 2 µg/ml
  
  Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
  Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
  Lane 3 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
  
  Observed band size: 48 kDawhy is the actual band size different from the predicted?
  
  

• 

  Flow Cytometry - Anti-EBP50/NHERF-1 antibody [EBP-10] (ab9526)

  Overlay histogram showing HepG2 cells stained with ab9526 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab9526, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.