Anti-PF4抗体
参阅全部 PF4 一抗
兔多克隆抗体to PF4
Rabbit
适用于: WB, Sandwich ELISAmore details
与反应: Human
Recombinant full length protein corresponding to Human PF4. Highly pure (>98%) recombinant hPF-4 (human Platelet Factor-4)
Database link: P02776
Recombinant human PF4 protein (ab9562) can be used as a positive control in WB.
This product is no longer batch tested in IHC, for an IHC validated antibody please see ab49735
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Lyophilized:Reconstitute with 200µl of sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Immunogen affinity purified
多克隆
unknown
unknown
Abpromise™承诺保证使用ab9561于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (1) | Use at an assay dependent concentration. Detects a band of approximately 11 kDa (predicted molecular weight: 11 kDa). To detect hPF-4 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hPF-4 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. Read More |
| Sandwich ELISA | Use at an assay dependent concentration. Can be paired for Sandwich ELISA with ab83123 |
Entrez Gene: 5196 Human
Omim: 173460 Human
SwissProt: P02776 Human
Unigene: 81564 Human
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Western blot - Anti-PF4 antibody (ab9561)
Anti-PF4 antibody (ab9561) at 1 µg/ml + Human Platelet (Human adult normal cell line) Whole Cell Lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 11 kDa
Observed band size: 11 kDa
Additional bands at: 18 kDa, 30 kDa, 37 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 2 minutes
Sandwich ELISA - Anti-PF4 antibody (ab9561)
To detect Human PF-4 by sandwich ELISA (using 100μl/well) a concentration of 0.5-2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with suitable detection antibody, allows the detection of at least 2000-4000 pg/ml of Recombinant Human PF-4.
