Anti-Elastin antibody [BA-4]

• 产品名称

  Anti-Elastin抗体[BA-4]
  参阅全部 Elastin 一抗

• 描述

  小鼠单克隆抗体[BA-4] to Elastin

• 宿主

  Mouse

• 经测试应用

  适用于: WBmore details
  不适用于: IHC-Fr or IHC-P

• 种属反应性

  与反应: Human
  预测可用于: Sheep, Goat, Guinea pig, Cat, Dog, Pig

• 免疫原

  The details of the immunogen for this antibody are not available.

• 表位

  BA-4 is specific for a chemotactically active epitope composed of valine, glycine, alanine, and proline in a molar ratio of approximately 2:2:1:1 (PMID 2429696).

• 阳性对照

• WB: Human skin tissue lysate.

• 常规说明

  
  

  This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.

• 存储溶液

  Preservative: 0.02% Sodium azide
  Constituent: PBS

• 浓度

• 100 µg 浓度为 1 mg/ml

• 纯度

  Affinity purified

• Primary antibody说明

  Elastin is an important polymeric protein of connective tissue that imparts elasticity to vertebrate elastic tissues.

• 克隆

  单克隆

• 克隆编号

  BA-4

• 骨髓瘤

  unknown

• 同种型

  IgG1

• 轻链类型

  unknown

The Abpromise guarantee

Abpromise™承诺保证使用ab9519于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用说明

Is unsuitable for IHC-Fr or IHC-P.

• 数据库链接

• Entrez Gene: 2006 Human

• Omim: 130160 Human

• SwissProt: P15502 Human

• Unigene: 647061 Human

• 别名

• SVAS antibody

• Tropoelastin antibody

• WBS antibody

• WS antibody

• Elastin antibody

• ELN antibody

• ELN_HUMAN antibody

• 

  Immunohistochemistry (Frozen sections) - Anti-Elastin antibody [BA-4] (ab9519)

  IHC image of ab9519 staining in 10% formaldehyde fixed frozen tissue section of normal human skin.

  Non-specific protein-protein interactions were blocked using TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 3% (w/v) BSA for 1h at room temperature. The section was then incubated with ab9519 (1μg/ml) in TBS containing 0.025% (v/v) Triton X-100 and 3% (w/v) BSA overnight at +4°C. The section was then incubated with ab150119 (Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 647)) and DAPI for 1 hour at room temperature.

  The DAPI only control (no antibody) inset shows no autofluorescence, demonstrating that any Alexa Fluor® 647 signal is derived directly from bound ab9519. 

  For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antibody concentrations and incubation times.

• 

  Western blot - Anti-Elastin antibody [BA-4] (ab9519)

  Anti-Elastin antibody [BA-4] (ab9519) at 0.51 µg/ml + Human skin tissue lysate - total protein (ab30166) at 20 µg
  
  Secondary
  Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
  
  Developed using the ECL technique.
  
  Performed under reducing conditions.
  
  Predicted band size: 68 kDa
  Observed band size: 70 kDawhy is the actual band size different from the predicted?
  Additional bands at: 55 kDa. We are unsure as to the identity of these extra bands.
  
  
  Exposure time: 20 minutes