Anti-VEGFC抗体
参阅全部 VEGFC 一抗
兔多克隆抗体to VEGFC
Rabbit
适用于: IHC-P, IHC-FoFr, WB, ELISAmore details
不适用于: IP
与反应: Mouse, Rat, Sheep, Human
Recombinant fragment corresponding to Rat VEGFC aa 101-221. Produced from sera of rabbits pre-immunized with highly pure recombinant rat VEGF-C (Asp101-Ile221) derived from insect cells.
Sequence:
DTVKLAAAHYNTEILKSIDNEWRKTQCMPREVCIDVGKEFGAATNTFFKP PCVSVYRCGGCCNSEGLQCMNTSTGYLSKTLFEITVPLSQGPKPVTISFA NHTSCRCMSKLDVYRQVHSIIHHHHHH
Database link: P49767
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Constituent: PBS
浓度
100 µg 浓度为 1 mg/ml
Protein A purified
多克隆
IgG
Abpromise™承诺保证使用ab9546于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | Use at an assay dependent concentration. PubMed: 21359148 | |
| IHC-FoFr | Use at an assay dependent concentration. PubMed: 22206010 | |
| WB | Use a concentration of 2 - 5 µg/ml. | |
| ELISA | Use a concentration of 1 - 5 µg/ml. Allows the detection of 0.5-1.0 ng/well of recombinant rat VEGF-C. |
应用说明
Is unsuitable for IP.
Entrez Gene: 7424 Human
Entrez Gene: 22341 Mouse
Omim: 601528 Human
SwissProt: P49767 Human
SwissProt: Q6FH59 Human
SwissProt: P97953 Mouse
Unigene: 435215 Human
Unigene: 1402 Mouse
Unigene: 6913 Rat
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Western blot - Anti-VEGFC antibody (ab9546)
Western analysis of recombinant human and rat VEGF-C using a polyclonal rabbit anti-rat VEGF-C antibody.
Western blot - Anti-VEGFC antibody (ab9546)
Western blot with recombinant rat VEGF C fused to His-tag using the polyclonal anti VEGF C antibody ab9546. Bands are between 15kDa and 20kDa represents glycosylation forms.
Western blot - Anti-VEGFC antibody (ab9546)
Western blot with human and rat VEGF C and VEGF D using the polyclonal anti VEGF C antibody ab9546. Proteins were separated by 15% SDS-PAGE and blotted on to PVDF membranes as described. After transfer, the membrane was incubated for 1 h with ab9546 at 1 µg/ml in TBS containing 20% non-fat milk followed by incubation with a goat-anti-rabbit alkaline phosphatase-conjugated secondary antibody. Lane 1/250ng rat VEGF C; lane 2/250 ng human VEGF C; lane 3/250 ng rat VEGF D; lane4/250 ng human VEGF-D.
Western blot - Anti-VEGFC antibody (ab9546)
Anti-VEGFC antibody (ab9546) + Rat Recombinant VEGF-C insect cells
Western Blot was run on a 15% SDS-PAGE gel. Bands between 15kDa and 20kDa represent the different glycoslation forms.
ELISA - Anti-VEGFC antibody (ab9546)
VEGF-C sandwich-ELISA using ab9546 as capture antibody and recombinant rat VEGF-C as standard. Biotinylated rabbit anti-rat VEGF-C was used for detection.
