Anti-CD74 antibody [LN2]

• 产品名称

  Anti-CD74抗体[LN2]
  参阅全部 CD74 一抗

• 描述

  小鼠单克隆抗体[LN2] to CD74

• 宿主

  Mouse

• 经测试应用

  适用于: Flow Cyt, WB, IHC-P, Flow Cyt (Intra)more details

• 种属反应性

  与反应: Human
  

• 免疫原

  Tissue, cells or virus corresponding to Human CD74. SU-DHL-4 lymphoma cells

• 阳性对照

• Human tonsil normal tissue lysate - total protein: Hum an tonsil and Human liver tissues; Raji cells

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  pH: 7.3
  Preservative: 0.1% Sodium azide
  Constituent: 1% BSA

• 纯度

  Protein A/G purified

• 克隆

  单克隆

• 克隆编号

  LN2

• 骨髓瘤

  unknown

• 同种型

  IgG1

• 轻链类型

  kappa

The Abpromise guarantee

Abpromise™承诺保证使用ab9514于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 972 Human

• Omim: 142790 Human

• SwissProt: P04233 Human

• Unigene: 436568 Human

• 别名

• CD74 antigen antibody

• CD74 molecule antibody

• CD74 molecule, major histocompatibility complex, class II invariant chain antibody

• CLIP antibody

• DHLAG antibody

• Gamma chain of class II antigens antibody

• HG2A_HUMAN antibody

• HLA class II histocompatibility antigen gamma chain antibody

• HLA DR antigens associated invariant chain antibody

• HLA DR gamma antibody

• HLA-DR antigens-associated invariant chain antibody

• HLA-DR-gamma antibody

• HLADG antibody

• HLADR antigens associated invariant chain antibody

• Ia antigen associated invariant chain antibody

• Ia antigen-associated invariant chain antibody

• Ia associated invariant chain antibody

• Ia gamma antibody

• Ii antibody

• Invariant polypeptide of major histocompatibility complex class II antigen associated antibody

• la-gamma antibody

• Major histocompatibility complex class II invariant chain antibody

• MHC HLA DR gamma chain antibody

• MHC HLA-DR gamma chain antibody

• p33 antibody

• p35 antibody

• Protein 41 antibody

• CD 74 antibody

• CD74 antibody

• CD74 antigen (invariant polypeptide of major histocompatibility complex, class II antigen-associated) antibody

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD74 antibody [LN2] (ab9514)

  Formalin-fixed, paraffin-embeded human tonsil tissue stained for CD74 using ab9514 at 1/50 dilution in immunohistochemical analysis. Antigen retrieval with citrate buffer pH 6.0.

• 

  Flow Cytometry (Intracellular) - Anti-CD74 antibody [LN2] (ab9514)

  Flow cytometry overlay histogram showing wild-type Raji (green line) and CD74 knockout Raji cells (ab273378) stained with ab9514 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS containing 10μg/ml human IgG and 10% normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab9514) (1x106 in 100μl at 1 μg/ml) for 30 min at 22°C.

  The secondary antibody Goat anti-mouse IgG H&L (Alexa Fluor® 488, pre-adsorbed) (ab150117) was used at 1/2000 for 30 min at 22°C.

  Isotype control antibody was mouse IgG1κ (ab170190) used at the same concentration and conditions as the primary antibody (wild-type Raji cells - black line; CD74 knockout Raji cells ab273378 - grey line). Unlabelled sample was also used as a control (this line is not shown for the purpose of simplicity).

  Acquisition of >5000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter.

  This antibody can also be used in Raji cells fixed with 4% formaldehyde (10 min) / permeabilized with 0.1% PBS-Triton X-100 for 15 min used under the same conditions.

   

• 

  Flow Cytometry - Anti-CD74 antibody [LN2] (ab9514)

  Overlay histogram showing Raji cells stained with ab9514 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab9514, 1/10 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
  Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

• 

  Western blot - Anti-CD74 antibody [LN2] (ab9514)

  Anti-CD74 antibody [LN2] (ab9514) at 5 µg/ml + Human tonsil normal tissue lysate - total protein (ab29615) at 10 µg
  
  Secondary
  Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
  
  Predicted band size: 34 kDa
  Observed band size: 34 kDa
  Additional bands at: 37 kDa (possible post-translational modification)
  
  

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD74 antibody [LN2] (ab9514)

  Ab9514 staining CD74 in paraffin embedded Human tonsil tissue sections by Immunohistochemistry (IHC-P).

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD74 antibody [LN2] (ab9514)Image courtesy of an anonymous Abreview.

  ab9514 staining CD74 in human liver tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with neutral buffered formalin and a heat mediated antigen retrieval step was performed using EDTA buffer pH 9.0. Samples were then blocked with 10% serum for 10 minutes at 20°C followed by incubation with the primary antibody at a 1/75 dilution for 30 minutes at 20°C. A HRP-conjugated rat anti-mouse/rabbit polyclonal was used undiluted as the secondary antibody.

  See Abreview