Anti-PLGF antibody - N-terminal

• 产品名称

  Anti-PLGF抗体- N-terminal
  参阅全部 PLGF 一抗

• 描述

  兔多克隆抗体to PLGF - N-terminal

• 宿主

  Rabbit

• 经测试应用

  适用于: WB, ICC/IFmore details

• 种属反应性

  与反应: Human, Recombinant fragment
  

• 免疫原

  Synthetic peptide corresponding to Human PLGF (N terminal). Highly purified N-terminal 20 aa synthetic peptide (Human).
  Database link: P49763

• 常规说明

  According to Swissprot P49763; PLGF exists in three isoforms PLGF 1, 2 and 3. The N-terminal immunogen sequence of ab9542 is present in all isoforms so extra bands might be observed near the band of interest.

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Lyophilized:Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  Constituent: PBS

• 纯度

  Protein A purified

• 克隆

  多克隆

• 同种型

  IgG

The Abpromise guarantee

Abpromise™承诺保证使用ab9542于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 5228 Human

• Omim: 601121 Human

• SwissProt: P49763 Human

• Unigene: 252820 Human

• 别名

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• PlGF antibody

• PLGF_HUMAN antibody

• PlGF2 antibody

• SHGC 10760 antibody

• D12S1900 antibody

• Pgf antibody

• PGFL antibody

• 

  Western blot - Anti-PLGF antibody - N-terminal (ab9542)

  Western blot of chromatography purified, recombinant PLGF-1/-2 and VEGF165 immobilized to PVDF-membrane.
  PlGF antibody shows no cross-reactivity to VEGF165.

• 

  Immunocytochemistry/ Immunofluorescence - Anti-PLGF antibody - N-terminal (ab9542)

  ICC/IF image of ab9542 stained HepG2 (human liver hepatocellular carcinoma cell line) cells. The cells were fixed in 4% formaldehyde for 10 minutes and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1 hour to permeabilize the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab9542, 1 µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1 hour. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1 hour. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43 µM.