Anti-Phosphotyrosine antibody

• 产品名称

  Anti-Phosphotyrosine抗体
  参阅全部 Phosphotyrosine 一抗

• 描述

  兔多克隆抗体to Phosphotyrosine

• 宿主

  Rabbit

• 特异性

  Recognize proteins and peptides phosphorylated on tyrosine residues. No cross-reaction with phosphoserine and threonine.

• 经测试应用

  适用于: ELISA, IP, WB, IHC-P, IHC-Frmore details

• 种属反应性

  与反应: Species independent
  

• 免疫原

  Chemical/ Small Molecule corresponding to Phosphotyrosine conjugated to keyhole limpet haemocyanin. Phospho-tyrosine KLH conjugates

• 阳性对照

• Use Vanadium treated cell culture (30 min) for Western Blotting and Tyrosine-phosphorylated peptide for ELISA. Human breast cancer tissue

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.

• 存储溶液

  pH: 6.00
  Preservative: 0.01% Sodium azide
  Constituent: 0.114% Tris buffered saline

• 浓度

• 100 µg 浓度为 0.25 mg/ml

• 纯度

  Immunogen affinity purified

• 纯化说明

  Affinity purified with phospho-tyrosine on agarose and competitively eluted with N-acetylated phospho-tyrosine.

• 克隆

  多克隆

• 同种型

  IgG

The Abpromise guarantee

Abpromise™承诺保证使用ab9319于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 

  Western blot - Anti-Phosphotyrosine antibody (ab9319)

  All lanes : Anti-Phosphotyrosine antibody (ab9319) at 0.5 µg/ml
  
  Lane 1 : 5 ul of crude protein from of HeLa cell lysate treated with fetal calf serum
  Lane 2 : 15 ul of crude protein from HeLa cell lysate treated with fetal calf serum
  Lane 3 : 5 ul of crude protein from HeLa cell lysate
  Lane 4 : 15 ul of crude protein from HeLa cell lysate
  
  Lysates/proteins at 2 mg/ml per lane.
  

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phosphotyrosine antibody (ab9319)

  Immunohistochemistry analysis of human breast cancer with ab9319