生物素Anti-Phosphothreonine抗体
参阅全部 Phosphothreonine 一抗
生物素兔多克隆抗体to Phosphothreonine
Rabbit
Biotin
Reacts with free phosphothreonine but does not react with phosphoserine, threonine or phosphotyrosine.
适用于: WB, IP, ELISAmore details
与反应: Species independent
Chemical/ Small Molecule corresponding to Phosphothreonine conjugated to keyhole limpet haemocyanin.
Use mouse brain extract for immunoblotting. Use synthetic phosphopeptide (on threonine) for ELISA.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 6
Preservative: 0.02% Sodium azide
浓度
100 µg 浓度为 0.25 mg/ml
Immunogen affinity purified
Immunoaffinity chromatography with phosphothreonine-agarose.
多克隆
IgG
Abpromise™承诺保证使用ab9340于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | ||
| IP | ||
| ELISA |
应用说明
ELISA(kinase assay): Use at 0.5 µg/mL
Western blot: Use at 4µg/mL
IP: Use at 10 µg/250 µg protein sample
Will detect 100 ng of phosvitin in Western Blots and 0.5 ng of phosvitin with ELISA.
Can be used for non-radioactive protein kinase assay (ELISA) using biotinylated peptide substrate and immunoblotting of abundant phosphoproteins.
It is not recommended for immunoblotting of trace cellular phosphoproteins. Acetone precipitation of the protein extract followed by SDS denaturation is recommended for successful immunoprecipitation.
Western blot - Biotin Anti-Phosphothreonine antibody (ab9340)
Immunoblotting of fetal mouse brain extract (125 ug - A and 25 ug - B)
Immunoblotting of fetal mouse brain extract (125 ug - A and 25 ug - B)
ELISA - Biotin Anti-Phosphothreonine antibody (ab9340)
Antibody Capture ELISA
Label: immobilized antigen
