Anti-Cytokeratin 18 antibody [RGE53]

• 产品名称

  Anti-Cytokeratin 18抗体[RGE53]
  参阅全部 Cytokeratin 18 一抗

• 描述

  小鼠单克隆抗体[RGE53] to Cytokeratin 18

• 宿主

  Mouse

• 特异性

  This antibody reacts exclusively with cytokeratin 18 which is present in glandular epithelial cells of the digestive, respiratory, and urogenital tracts, endocrine and exocrine cells and mesothelial cells, as well as adenocarcinomas originating from them

• 经测试应用

  适用于: Flow Cyt, IHC-Fr, WBmore details

• 种属反应性

  与反应: Human, Pig, Zebrafish
  预测可用于: Mouse, Rat, Rabbit, Chicken, Hamster, Dog

• 免疫原

  Full length protein corresponding to Human Cytokeratin 18. Cytoskeletal preparation of HeLa cells.

• 阳性对照

• This antibody gave a positive signal in HeLa and MCF7 whole cell lysates.

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  Preservative: 0.09% Sodium azide
  Constituent: PBS

• 浓度

• 100 µg 浓度为 1 mg/ml

• 纯度

  Protein G purified

• 克隆

  单克隆

• 克隆编号

  RGE53

• 骨髓瘤

  unknown

• 同种型

  IgG1

The Abpromise guarantee

Abpromise™承诺保证使用ab9217于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

数据库链接

• Entrez Gene: 477601 Dog

• Entrez Gene: 3875 Human

• Entrez Gene: 16668 Mouse

• Entrez Gene: 100126286 Pig

• Entrez Gene: 294853 Rat

• Entrez Gene: 352912 Zebrafish

• Omim: 148070 Human

• SwissProt: P05783 Human

• SwissProt: P05784 Mouse

• SwissProt: Q5BJY9 Rat

• SwissProt: Q7ZTS4 Zebrafish

• Unigene: 406013 Human

• Unigene: 22479 Mouse

• Unigene: 103924 Rat

• Unigene: 39128 Zebrafish

别名

• Cell proliferation inducing gene 46 protein antibody

• Cell proliferation inducing protein 46 antibody

• Cell proliferation-inducing gene 46 protein antibody

• CK 18 antibody

• CK-18 antibody

• CK18 antibody

• CYK 18 antibody

• CYK18 antibody

• Cytokeratin 18 antibody

• Cytokeratin endo B antibody

• Cytokeratin-18 antibody

• K 18 antibody

• K18 antibody

• K1C18_HUMAN antibody

• KA18 antibody

• Keratin 18 antibody

• Keratin 18, type I antibody

• Keratin D antibody

• keratin, type I cytoskeletal 18 antibody

• Keratin-18 antibody

• Krt18 antibody

• 

  Western blot - Anti-Cytokeratin 18 antibody [RGE53] (ab9217)

  All lanes : Anti-Cytokeratin 18 antibody [RGE53] (ab9217) at 5 µg/ml
  
  Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
  Lane 2 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
  
  Lysates/proteins at 20 µg per lane.
  
  Secondary
  All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
  
  Developed using the ECL technique.
  
  Performed under reducing conditions.
  
  Predicted band size: 48 kDa
  Observed band size: 48 kDa
  
  
  Exposure time: 30 seconds
  

• 

  Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 18 antibody [RGE53] (ab9217)

  Immunohistochemistry on frozen section of human kidney epithelium.

• 

  Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 18 antibody [RGE53] (ab9217)

  Immunofluorescence staining images of 9 day old zebrafish embryos.
  ab9217 reacts with with intestinal epithelium and epidermis. Frozen sample treated with Acetone:Methanol 1:1, antibody diluted 1/100 and incubated for 45 minutes at room temperature.0

• 

  Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 18 antibody [RGE53] (ab9217)

  immunofluorescence staining of epithelial tissues in a 2 days old zebrafish embryo.

• 

  Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 18 antibody [RGE53] (ab9217)

  Immunofluorescence staining of 1 month old zebrafish embryo.

• 

  Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 18 antibody [RGE53] (ab9217)

  Immunofluorescence staining images of 9 day old zebrafish embryos.
  ab9217 reacts with intestinal epithelium and epidermis. Frozen sample treated with Acetone:Methanol 1:1, antibody diluted 1/100 and incubated for 45 minutes at room temperature.

• 

  Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 18 antibody [RGE53] (ab9217)

  Immunohistochemistry on frozen section of human kidney epithelium

• 

  Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 18 antibody [RGE53] (ab9217)

  Immunohistochemistry on frozen section of human colon

• 

  Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 18 antibody [RGE53] (ab9217)

  Immunohistochemistry on frozen section of swine liver hepatocytes

• 

  Flow Cytometry - Anti-Cytokeratin 18 antibody [RGE53] (ab9217)

  Overlay histogram showing MCF7 cells stained with ab9217 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab9217, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in MCF7 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.