Anti-Cytokeratin 14 antibody [RCK107]

• 产品名称

  Anti-Cytokeratin 14抗体[RCK107]
  参阅全部 Cytokeratin 14 一抗

• 描述

  小鼠单克隆抗体[RCK107] to Cytokeratin 14

• 宿主

  Mouse

• 特异性

  This antibody reacts exclusively with cytokeratin 14 which is present in basal cell compartments of stratified and combined epithelia.

• 经测试应用

  适用于: IHC-Fr, WB, ICC/IFmore details

• 种属反应性

  与反应: Dog, Human, Pig
  

• 免疫原

  Tissue, cells or virus corresponding to Human Cytokeratin 14. Cytoskeletal preparation of TR146 epithelial cells.

• 阳性对照

• cytoskeletal preparation of TR146 epithelial cells, skin tissue lysate (Human)

• 常规说明

   

  Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 – 7.8. The individual human cytokeratins are designated 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology assay.

  
  

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• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  Preservative: 0.09% Sodium azide
  Constituent: PBS

• 浓度

• 100 µg 浓度为 1 mg/ml

• 纯度

  Protein A purified

• Primary antibody说明

  Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 – 7.8. The individual human cytokeratins are designated 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.

• 克隆

  单克隆

• 克隆编号

  RCK107

• 骨髓瘤

  Sp2/0-Ag14

• 同种型

  IgG1

The Abpromise guarantee

Abpromise™承诺保证使用ab9220于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 3861 Human

• Omim: 148066 Human

• SwissProt: P02533 Human

• Unigene: 654380 Human

• 别名

• Cytokeratin 14 antibody

• Cytokeratin-14 antibody

• Cytokeratin14 antibody

• Dowling Meara antibody

• EBS3 antibody

• EBS4 antibody

• Epidermolysis bullosa simplex antibody

• K14 antibody

• K1C14_HUMAN antibody

• Keratin 14 (epidermolysis bullosa simplex, Dowling-Meara, Koebner) antibody

• Keratin 14 antibody

• Keratin antibody

• Keratin type I cytoskeletal 14 antibody

• Keratin, type I cytoskeletal 14 antibody

• Keratin-14 antibody

• Keratin14 antibody

• Koebner antibody

• Krt 14 antibody

• Krt14 antibody

• NFJ antibody

• OTTHUMP00000164624 antibody

• type I cytoskeletal 14 antibody

• CK 14 antibody

• CK-14 antibody

• ck14 antibody

• 

  Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 14 antibody [RCK107] (ab9220)

  Immunohistochemical analysis of swine skin tissue labeling Cytokeratin 14 with ab9220. Showing staining of basal cells.

• 

  Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 14 antibody [RCK107] (ab9220)

  ICC/IF image of ab9220 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab9220, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
  
  

• 

  Western blot - Anti-Cytokeratin 14 antibody [RCK107] (ab9220)

  Anti-Cytokeratin 14 antibody [RCK107] (ab9220) at 4 µg/ml + Human skin tissue lysate - total protein (ab30166) at 20 µg
  
  Secondary
  Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
  
  Developed using the ECL technique.
  
  Performed under reducing conditions.
  
  Observed band size: 58 kDawhy is the actual band size different from the predicted?
  Additional bands at: 72 kDa. We are unsure as to the identity of these extra bands.
  
  
  Exposure time: 20 minutes
  

• 

  Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 14 antibody [RCK107] (ab9220)

  Immunohistochemical analysis of dog skin epidermis tissue labelling Cytokeratin 14 using ab9220. Staining of basal keratinocyte layer is observed.

• 

  Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 14 antibody [RCK107] (ab9220)

  Immunohistochemical analysis of dog skin appendages labelling Cytokeratin 14 with ab9220.