Anti-CD59 antibody [MEM-43/5]

• 产品名称

  Anti-CD59抗体[MEM-43/5]
  参阅全部 CD59 一抗

• 描述

  小鼠单克隆抗体[MEM-43/5] to CD59

• 宿主

  Mouse

• 特异性

  CD59 antigen (human). MEM-43/5 reacts with well defined epitope (around L33) and does not compete with MEM-43 and many other CD59 antibodies

• 经测试应用

  适用于: ICC/IF, IP, IHC-P, Flow Cyt, WBmore details

• 种属反应性

  与反应: Mouse, Human
  

• 免疫原

  Tissue, cells or virus corresponding to Human CD59. Thymocytes and T lymphocytes

• 表位

  The antibody MEM-43/5 reacts with well defined epitope around L33 (see Bodian et al)

• 阳性对照

• Flow cyt: blood Jeg3 cell line IF/ICC

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  pH: 7.40
  Preservative: 0.097% Sodium azide
  Constituent: PBS

• 浓度

• 100 µg 浓度为 1 mg/ml

• 纯度

  Protein A purified

• 纯化说明

  Purity >95% by SDS-PAGE.

• 克隆

  单克隆

• 克隆编号

  MEM-43/5

• 骨髓瘤

  unknown

• 同种型

  IgG2b

• 轻链类型

  unknown

The Abpromise guarantee

Abpromise™承诺保证使用ab9183于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 966 Human

• Entrez Gene: 12509 Mouse

• Omim: 107271 Human

• SwissProt: P13987 Human

• SwissProt: O55186 Mouse

• Unigene: 278573 Human

• Unigene: 709466 Human

• Unigene: 710641 Human

• 别名

• 20 kDa homologous restriction factor antibody

• CD 59 antibody

• CD_antigen=CD59 antibody

• CD59 antibody

• CD59 antigen antibody

• CD59 antigen complement regulatory protein antibody

• CD59 antigen p18 20 antibody

• CD59 antigen p18-20 (antigen identified by monoclonal antibodies 16.3A5, EJ16, EJ30, EL32 and G344) antibody

• CD59 glycoprotein antibody

• CD59 molecule antibody

• CD59 molecule complement regulatory protein antibody

• CD59_HUMAN antibody

• Cd59a antibody

• Complement regulatory protein antibody

• EJ16 antibody

• EJ30 antibody

• EL32 antibody

• FLJ38134 antibody

• FLJ92039 antibody

• G344 antibody

• HRF 20 antibody

• HRF-20 antibody

• HRF20 antibody

• Human leukocyte antigen MIC11 antibody

• Ly 6 like protein antibody

• Lymphocytic antigen CD59/MEM43 antibody

• MAC inhibitory protein antibody

• MAC IP antibody

• MAC-inhibitory protein antibody

• MAC-IP antibody

• MACIF antibody

• MACIP antibody

• MEM43 antibody

• MEM43 antigen antibody

• Membrane attack complex (MAC) inhibition factor antibody

• Membrane attack complex inhibition factor antibody

• Membrane inhibitor of reactive lysis antibody

• MGC2354 antibody

• MIC11 antibody

• MIN1 antibody

• MIN2 antibody

• MIN3 antibody

• MIRL antibody

• MSK21 antibody

• p18 20 antibody

• Protectin antibody

• Surface antigen recognized by monoclonal antibody 16.3A5 antibody

• T cell activating protein antibody

• 16.3A5 antibody

• 1F5 antibody

• 1F5 antigen antibody

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD59 antibody [MEM-43/5] (ab9183)

  Immunohistochemistry parafin embedded sections staining of huam skin tissue using ab9183.

• 

  Flow Cytometry - Anti-CD59 antibody [MEM-43/5] (ab9183)

  Flow cytometric analysis of Human Peripheral Blood cells labelling CD59 with ab9183 at 0.6 ug/ml showing separation of human neutrophil granulocytes (red-filled) from human CD59 negative blood debris (black-dashed).

• 

  Immunocytochemistry/ Immunofluorescence - Anti-CD59 antibody [MEM-43/5] (ab9183)

  ICC/IF image of ab9183 stained Jeg3 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab9183, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD59 antibody [MEM-43/5] (ab9183)

  IHC image of ab9183 staining CD59 in Human normal placenta formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH9, epitope retrieval solution 2) for 20 mins. The section was then incubated with ab9183, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  
  For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

• 

  Flow Cytometry - Anti-CD59 antibody [MEM-43/5] (ab9183)

  Overlay histogram showing Jurkat cells stained with ab9183 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab9183, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.