Anti-SHP2抗体
参阅全部 SHP2 一抗
山羊多克隆抗体to SHP2
Goat
适用于: WB, ICC/IF, Flow Cyt (Intra)more details
与反应: Human
预测可用于: Rat, Cow, Pig
Synthetic peptide corresponding to Human SHP2 aa 550 to the C-terminus (C terminal). (NP_001317366.1)
Database link: NP_002825.3
WB: Human muscle lysate. ICC/IF: HeLa cells Flow Cyt (intra): A431 cells
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Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA
浓度
100 µg 浓度为 0.5 mg/ml
Immunogen affinity purified
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
多克隆
IgG
Abpromise™承诺保证使用ab9214于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 68 kDa). 1 hour primary incubation is recommended for this product. | |
| ICC/IF | (1) | Use a concentration of 10 µg/ml. |
| Flow Cyt (Intra) | Use a concentration of 10 µg/ml. |
Entrez Gene: 5781 Human
Omim: 176876 Human
SwissProt: Q06124 Human
Unigene: 506852 Human
Unigene: 98209 Rat
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Immunocytochemistry/ Immunofluorescence - Anti-SHP2 antibody (ab9214)
Immunocytochemistry/Immunofluorescence analysis of paraformaldehyde-fixed, 0.15% triton-permeabilized HeLa cells staining SHP2 antibody with ab9214 at 10µg/ml, followed by Alexa Fluor 488 secondary antibody at 2ug/ml. DAPI was used as a nuclear counterstain.
Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody at 2ug/ml
Flow Cytometry (Intracellular) - Anti-SHP2 antibody (ab9214)
Flow cytometric analysis of paraformaldehyde-fixed, 0.5% Triton-permeabilized A431 cells (blue) staining SHP2 with ab9214 at 10µg/ml, followed by Alexa Fluor 488 secondary antibody at 1ug/ml. Primary incubation carried out for one hour.
IgG control: Unimmunized goat IgG followed by Alexa Fluor 488 secondary antibody (black).
Western blot - Anti-SHP2 antibody (ab9214)
Anti-SHP2 antibody (ab9214) at 2 µg/ml + Human muscle lysate at 35 µg
Predicted band size: 68 kDa
Western blot - Anti-SHP2 antibody (ab9214)
ab9214 staining (2µg/ml) of Human Muscle lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. ab9214 staining (2µg/ml) of Human Muscle lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
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