Anti-NCAM1 antibody [RNL-1]

• 产品名称

  Anti-NCAM1抗体[RNL-1]
  参阅全部 NCAM1 一抗

• 描述

  小鼠单克隆抗体[RNL-1] to NCAM1

• 宿主

  Mouse

• 特异性

  Neural cell adhesion molecule (NCAM). ab9018 reacts with three isoforms of NCAM: NCAM-120, NCAM-140, and NCAM-180.

• 经测试应用

  适用于: ICC, IHC-FoFrmore details
  不适用于: Flow Cyt

• 种属反应性

  与反应: Rat, Human
  

• 免疫原

  Tissue, cells or virus corresponding to Human NCAM1. This antibody was raised against the small cell lung cancer cell line NCI-H82.
  Database link: P13591

• 阳性对照

• IHC-P: Mouse brain tissue. IHC-Fr: Rat brain tissue. ICC/IF: Adult-derived human liver stem cells and hepatic stellate cells.

• 常规说明

  Punctuate cytoplasmic staining is observed with ab9018. Some staining of red blood cells observed.

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  Preservative: 0.09% Sodium azide
  Constituent: PBS

• 纯度

  Protein G purified

• 克隆

  单克隆

• 克隆编号

  RNL-1

• 骨髓瘤

  Sp2/0-Ag14

• 同种型

  IgG1

The Abpromise guarantee

Abpromise™承诺保证使用ab9018于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用说明

Is unsuitable for Flow Cyt.

• 数据库链接

• Entrez Gene: 4684 Human

• Entrez Gene: 24586 Rat

• Omim: 116930 Human

• SwissProt: P13591 Human

• SwissProt: P13596 Rat

• Unigene: 503878 Human

• Unigene: 11283 Rat

• 别名

• cell adhesion molecule, neural, 1 antibody

• MSK 39 antibody

• MSK39 antibody

• N-CAM-1 antibody

• NCAM 1 antibody

• NCAM antibody

• NCAM C antibody

• NCAM-1 antibody

• NCAM1 antibody

• NCAM1_HUMAN antibody

• NCAMC antibody

• Neural cell adhesion molecule 1 antibody

• Neural cell adhesion molecule NCAM antibody

• OTTHUMP00000235666 antibody

• antigen MSK39 identified by monoclonal antibody 5.1H11 antibody

• antigen recognized by monoclonal antibody 5.1H11 antibody

• CD56 antibody

• 

  Immunocytochemistry - Anti-NCAM1 antibody [RNL-1] (ab9018)

  Immunohistochemistry of H82 cells, showing staining for NCAM1 using ab9018 (1/500).

• 

  Immunocytochemistry - Anti-NCAM1 antibody [RNL-1] (ab9018)Image cropped from Berardis et al., Public Library of Science One, 1, e86137, Fig. 3a.; DOI: 10.1371/journal.pone.0086137 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

  Immunocytochemical analysis of Adult-Derived Human Liver Stem Cells (ADHLSC) in the left image and Hepatic Stellate Cells (HSC) in the right image. ab9018 was used to label NCAM, which is expressed in the HSC. Cells were fixed in 3.5% paraformaldehyde for 15 minutes at room temperature. Blocking was with 3.3% hydrogen peroxide for 3 minutes. Permeabilization with D-PBS and 1% Triton X-100 for 10 minutes. Cells incubated with ab9018 at 1/100 for 1 hour. DAB staining. Counterstaining with Mayer's hematoxylin. 

• 

  Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-NCAM1 antibody [RNL-1] (ab9018)

  ab9018 at a dilution of 1/500, staining NCAM (Alexa 488 secondary at 1/2000) on rat brain tissue (30µm thick coronal sections) in free floating IHC (see protocol link for detailed description). Images showing neuron body and processes: [A] 40x objective and [B] punctate cytoplasmic staining; 20x objective and [C] neuron + processes; x20 objective. No labeling observed following omission of primary antibody. Images coloured in Photoshop.

  NB: We recommend indirect amplification of immunofluorescence for ab9018

  Sections were viewed using an Axioplan 2 Imaging microscope (Imaging Associates) fitted with 10x, 20x and 40x Plan-Neofluorobjectives (Zeiss, Germany) and images were taken using a AxioCam Hrm digital camera (Zeiss, Germany) and AxioVision software (Imaging Associates).