Anti-Cytokeratin 7抗体[RCK105] - Cytoskeleton Marker
参阅全部 Cytokeratin 7 一抗
小鼠单克隆抗体[RCK105] to Cytokeratin 7 - Cytoskeleton Marker
Mouse
This antibody reacts exclusively with cytokeratin 7 which is present in a subgroup of glandular epithelia and their tumors, as well as transitional epithelium and transitional carcinoma.
适用于: IHC-Fr, IHC-Pmore details
与反应: Human, Pig
预测可用于: Rat, Goat, Hamster, Cat, Dog, Zebrafish
Full length native protein (purified) corresponding to Human Cytokeratin 7. BALB/c mouse immunized with cytokeratins from the human bladder carcinoma cell line T24.
IHC-P: Human liver tissue, mouse ovary and bladder tissue IHC-Fr: Human liver, urinary bladder tissue, Pig liver and skin tissue
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.09% Sodium azide
Constituent: PBS
浓度
100 µg 浓度为 1 mg/ml
Protein G purified
单克隆
RCK105
Sp2/0-Ag14
IgG1
Abpromise™承诺保证使用ab9021于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
IHC-Fr | (2) | 1/100 - 1/500. |
IHC-P | (5) | 1/1000. |
Entrez Gene: 3855 Human
Omim: 148059 Human
SwissProt: P08729 Human
Unigene: 411501 Human
Unigene: 670221 Human
Unigene: 7913 Rat
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [RCK105] - Cytoskeleton Marker (ab9021)
Immunohistochemistry analysis of formalin fixed, paraffin embedded sections of human liver tissue labeling Cytokeratin 7 with ab9021 at 1/100; showing positive staining in the epithelial cells lining the bile ducts and no reactivity in hepatocytes or connective tissue.
Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 7 antibody [RCK105] - Cytoskeleton Marker (ab9021)
ab9021 staining normal pig skin tissue section (frozen), staining epithelial cells using ab9021 at 1/500.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [RCK105] - Cytoskeleton Marker (ab9021)Yang, X. et al PLoS One. 2013 Sep 18;8(9):e74809. doi: 10.1371/journal.pone.0074809. eCollection 2013 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/
Immunofluorescent staining of β-galactosideasae and cytokeratin expression of urinary bladder after intravesical treatment with Adeno-Cre
Formalin-fixed, paraffin-embedded mouse bladder tissue stained for β-galactosidase (green, A) or Cytokeratin 7 (red, B). Panel C shows the colocalization of β-galactosidase and Cytokeratin 7. The nuclear counterstain is DAPI (blue).
Cytokeratin 7 was detected using ab9021.
(After Figure 3 of Yang et al)
Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 7 antibody [RCK105] - Cytoskeleton Marker (ab9021)
ab9021 staining normal breast tissue section (frozen), fixated in Methanol, staining epithelial cells. These pictures are part of the review submitted by Rene Villadsen.
Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 7 antibody [RCK105] - Cytoskeleton Marker (ab9021)
Immunohistochemistry analysis of frozen sections of human liver labeling Cytokeratin 7 with ab9021 at 1/500; showing positive staining in the epithelial cells lining the bile ducts and no reactivity in hepatocytes or connective tissue.
Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 7 antibody [RCK105] - Cytoskeleton Marker (ab9021)
Immunohistochemistry analysis of frozen sections of human urinary bladder labeling Cytokeratin 7 with ab9021 at 1/500; showing positive staining in the epithelial cells and no reactivity in connective tissue.
Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 7 antibody [RCK105] - Cytoskeleton Marker (ab9021)
Immunohistochemistry on frozen sections of human liver, only bile duct epithelium positive; no reaction in hepatocytes
Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 7 antibody [RCK105] - Cytoskeleton Marker (ab9021)
Immunohistochemistry on frozen sections of swine liver, only bile duct epithelium positive; no reaction in hepatocytes
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [RCK105] - Cytoskeleton Marker (ab9021)This image is courtesy of an Abreview submitted by These pictures are part of the review submitted by Todd Gocken
Formalin fixed parrafin embedded mouse ovary tissue. Ab9021 used at 1:1000 incubated with overnight incubation with a biotinylated secondary and peroxidase/DAB detection.