Anti-CD11b antibody [M1/70]

• 产品名称

  Anti-CD11b抗体[M1/70]
  参阅全部 CD11b 一抗

• 描述

  大鼠单克隆抗体[M1/70] to CD11b

• 宿主

  Rat

• 经测试应用

  适用于: ICC/IF, Flow Cytmore details
  不适用于: IHC-P

• 种属反应性

  与反应: Mouse, Human
  

• 免疫原

  Tissue, cells or virus. This information is proprietary to Abcam and/or its suppliers.

• 阳性对照

• Flow Cytometry: RAW 264.7 cells, Human peripheral blood mononuclear cell (PBMC), Mouse peripheral blood mononuclear cell (PBMC) IF/ICC: RAW 264.7 cells

• 常规说明

  
  

  This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

  This product is a recombinant monoclonal antibody, which offers several advantages including:

  For more information see here.

• - High batch-to-batch consistency and reproducibility

• - Improved sensitivity and specificity

• - Long-term security of supply

• - Animal-free production

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

• 存储溶液

  pH: 7.2
  Preservative: 0.01% Sodium azide
  Constituents: 59% PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine)

• 浓度

• 100 µg 浓度为 1 mg/ml

• 纯度

  Protein A purified

• 克隆

  单克隆

• 克隆编号

  M1/70

• 骨髓瘤

  NS1

• 同种型

  IgG2b

• 轻链类型

  unknown

The Abpromise guarantee

Abpromise™承诺保证使用ab8878于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用说明

Is unsuitable for IHC-P.

• 数据库链接

• Entrez Gene: 3684 Human

• Entrez Gene: 16409 Mouse

• Omim: 120980 Human

• SwissProt: P11215 Human

• SwissProt: P05555 Mouse

• Unigene: 172631 Human

• Unigene: 262106 Mouse

• 别名

• CD11 antigen-like family member B antibody

• CD11b antibody

• CD11b/CD18 antibody

• CD49d antibody

• Cell surface glycoprotein MAC-1 subunit alpha antibody

• Complement component 3 receptor 3 subunit antibody

• Complement Component Receptor 3 Alpha antibody

• Complement receptor type 3 antibody

• Complement receptor type 3, alpha subunit antibody

• CR 3 alpha chain (CR3A) antibody

• CR 3 alpha chain antibody

• CR-3 alpha chain antibody

• CR3 antibody

• CR3A antibody

• F730045J24Rik antibody

• Integrin Alpha M antibody

• Integrin alpha M chain antibody

• Integrin alpha-M antibody

• Integrin beta 2 alpha subunit antibody

• Integrin subunit alpha M antibody

• integrin, alpha M (complement component 3 receptor 3 subunit) antibody

• ITAM_HUMAN antibody

• ITGAM antibody

• Leukocyte adhesion receptor MO1 antibody

• Ly-40 antibody

• MAC 1 antibody

• Mac-1a antibody

• MAC1 antibody

• Mac1, alpha subunit antibody

• MAC1A antibody

• Macrophage antigen alpha polypeptide antibody

• MGC117044 antibody

• Mo1 antibody

• Mo1, alpha subunit antibody

• MO1A antibody

• Neutrophil adherence receptor alpha M subunit antibody

• Neutrophil adherence receptor antibody

• antigen CD11b (p170) antibody

• Antigen CD11b p170 antibody

• CD11 antigen like family member B antibody

• 

  Immunocytochemistry/ Immunofluorescence - Anti-CD11b antibody [M1/70] (ab8878)

  Confocal image showing membranous staining in RAW 264.7 cells.

  RAW 264.7, WEHI-231 and THP-1 cells were fixed in 4% PFA and permeabilized with 0.1% Triton X-100. Primary antibody, ab8878 at 1:50 was incubated overnight at 4° C, followed by AlexaFluor® 488- conjugated Goat anti-Rat secondary antibody (ab150157) at 1/1000 dilution at RT for 45 min. ab179513 Anti-beta Tubulin, used as a counterstain at 1/200 dilution, was co-incubated with ab8878 overnight at 4° C, followed by Alexa Fluor® 594 Goat Anti-Rabbit secondary (ab150080) at 1/1000 dilution at RT for 45 min. Nucleus were visualized using DAPI.

  Negative control: WEHI-231 (PMID: 2457584)

• 

  Flow Cytometry - Anti-CD11b antibody [M1/70] (ab8878)

  Flow Cytometry analysis of WEHI-231 (Mouse B cell lymphoma B lymphocyte, Left) / RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage, Right) cells stained for CD11b using AB8878 at a 1/1000 dilution (0.1 μg) (Red) compared to Rat monoclonal IgG - Isotype Control (Black) and cells without incubation with primary antibody and secondary antibody (Blue). Goat F(ab)2 Anti-Rat IgG Fc (Alexa Fluor® 488, ab150161) at 1/2000 dilution was used as the secondary antibody.

  Negative control: WEHI-231.(PMID: 2457584)

  Gated on viable cells.

• 

  Flow Cytometry - Anti-CD11b antibody [M1/70] (ab8878)

  Flow Cytometry analysis of Human peripheral blood mononuclear cell (PBMC) cells labelling CD11b with AB8878 at a 1/10,000 dilution (0.01 μg) (right) compared to Rat monoclonal IgG - Isotype Control (right). Goat F(ab)2 Anti-Rat IgG Fc (Alexa Fluor® 488, ab150161) at 1/2000 dilution was used as the secondary antibody.

  Gated on viable cells.

• 

  Flow Cytometry - Anti-CD11b antibody [M1/70] (ab8878)

  Flow Cytometry analysis of Mouse peripheral blood mononuclear cell (PBMC) cells labelling CD11b with AB8878 at a 1/10,000 dilution (0.01 μg) (right) compared to Rat monoclonal IgG - Isotype Control (right). Goat F(ab)2 Anti-Rat IgG Fc (Alexa Fluor® 488, ab150161) at 1/2000 dilution was used as the secondary antibody.

  Gated on viable cells.