Anti-Desmin抗体[D33]
参阅全部 Desmin 一抗
小鼠单克隆抗体[D33] to Desmin
Mouse
This antibody is highly reactive with desmin. On immunoblots only the 56 kD desmin band is stained. Non reactive with GFAP, Keratin, Vimentin and Neurofilament.
适用于: IHC-Pmore details
与反应: Human
Tissue, cells or virus. Immunogen derived from human Leiomyoma.
Database link: P17661
Sufficient antibody for 100-500 tests
This antibody stains muscle cells in human tissues
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
pH: 7.30
Preservative: 0.065% Sodium azide
Constituent: 99.93% PBS
Tissue culture supernatant
This antibody stains muscle cells in human tissues
单克隆
D33
IgG1
Abpromise™承诺保证使用ab8470于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | (6) | 1/100. |
Entrez Gene: 1674 Human
Omim: 125660 Human
SwissProt: P17661 Human
Unigene: 594952 Human
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmin antibody [D33] (ab8470)](https://www.abcam.cn/ps/products/8/ab8470/Images/ab8470-14247-anti-desmin-antibody-d33-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmin antibody [D33] (ab8470)
IHC image of ab8470 staining in human skeletal muscle formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab8470, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
