Anti-Desmin抗体
参阅全部 Desmin 一抗
兔多克隆抗体to Desmin
Rabbit
In immunoblots, this antibody reacts with a 53 kD polypeptide. It does not react with vimentin or human cytokeratins. This antibody can be used to distinguish rhabdomyosarcomas and leiomyosarcomas from other soft tissue tumors, lymphomas, and carcinomas.
适用于: WBmore details
不适用于: ICC/IF or IHC-P
与反应: Mouse, Rat, Human
Full length protein corresponding to Human Desmin.
Database link: P17661
Prolonged fixation in buffered formalin can destroy the epitope.
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
pH: 7.40
Preservative: 0.1% Sodium azide
Constituents: 0.01% PBS, 0.2% BSA
浓度
500 µl 浓度为 0.2 mg/ml
Protein G purified
多克隆
IgG
Abpromise™承诺保证使用ab8592于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (5) | Use at an assay dependent concentration. Detects a band of approximately 53 kDa. |
应用说明
Is unsuitable for ICC/IF or IHC-P.
Entrez Gene: 1674 Human
Entrez Gene: 13346 Mouse
Omim: 125660 Human
SwissProt: P17661 Human
SwissProt: P31001 Mouse
Unigene: 594952 Human
Unigene: 6712 Mouse
Unigene: 39196 Rat
CMD1I antibody
CSM1 antibody
CSM2 antibody
DES antibody
DESM_HUMAN antibody
Desmin antibody
FLJ12025 antibody
FLJ39719 antibody
FLJ41013 antibody
FLJ41793 antibody
Intermediate filament protein antibody
OTTHUMP00000064865 antibody
Western blot - Anti-Desmin antibody (ab8592)
All lanes : Anti-Desmin antibody (ab8592) at 1/1000 dilution
Lane 1 : RMS13 cell lysate
Lane 2 : A431 cell lysate
Lane 3 : Human skeletal muscle tissue lysate
Lane 4 : Human heart tissue lysate
Lane 5 : C2C12 cell lysate
Lane 6 : Mouse skeletal muscle tissue lysate
Lane 7 : Mouse heart tissue lysate
Lane 8 : L6 cell lysate
Lane 9 : Rat heart tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Observed band size: 53 kDawhy is the actual band size different from the predicted?
Exposure time: 3 minutes
Blocking and dilution buffer: 5% NFDM/TBST.
