Anti-Calcitonin抗体
参阅全部 Calcitonin 一抗
兔多克隆抗体to Calcitonin
Rabbit
适用于: Sandwich ELISA, IHC-P, WBmore details
与反应: Human
预测可用于: Mouse, Rat, Sheep, Horse, Dog, Pig
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Medullary carcinoma of the thyroid or thyroid c-cell hyperplasia.
Prolonged fixation in buffered formalin can destroy the epitope
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
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Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA
Immunogen affinity purified
多克隆
IgG
unknown
Abpromise™承诺保证使用ab8553于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
Sandwich ELISA | Use a concentration of 0.5 µg/ml. Can be paired for Sandwich ELISA with Mouse monoclonal [16B5] to Calcitonin (ab11493). For sandwich ELISA, use this antibody as Detection at 0.5µg/ml with ab11493 as Capture. | |
IHC-P | (2) | 1/300. Antigen retrieval is not essential but may optimise staining. |
WB | 1/200 - 1/500. Predicted molecular weight: 15 kDa. |
Entrez Gene: 796 Human
Entrez Gene: 12310 Mouse
Omim: 114130 Human
SwissProt: P01258 Human
SwissProt: P70160 Mouse
SwissProt: P01261 Sheep
CALC 1 antibody
CALC A antibody
CALC1 antibody
calcitonin 1 antibody
Calcitonin carboxyl-terminal peptide (CCP) antibody
CT antibody
katacalcin antibody
PDN-21 antibody
Sandwich ELISA - Anti-Calcitonin antibody (ab8553)
Standard Curve for Calcitonin dilution range 1pg/ml to 1ug/ml using Capture Antibody Mouse monoclonal [16B5] to Calcitonin (ab11493) at 5ug/ml and Detector Antibody Rabbit polyclonal to Calcitonin (ab8553) at 0.5ug/ml
Western blot - Anti-Calcitonin antibody (ab8553)
Anti-Calcitonin antibody (ab8553) at 1/25 dilution + Human thyroid lysate.
Predicted band size: 15 kDa
Observed band size: 14 kDawhy is the actual band size different from the predicted?
Additional bands at: 40 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcitonin antibody (ab8553)
Immunohistochemical analysis of human thyroid staining with ab8553.