Anti-EGFR antibody [2E9]

• 产品名称

  Anti-EGFR抗体[2E9]
  参阅全部 EGFR 一抗

• 描述

  小鼠单克隆抗体[2E9] to EGFR

• 宿主

  Mouse

• 特异性

  This antibody reacts with a protein determinant of the extra cellular domain of the human EGFR. Shows binding competition with EGF and reacts in immunoprecipitation with the functional EFR protein-tyrosine kinase complex. The antibody has no agonistic properties, but competes efficiently with EGF for binding to the receptor

• 经测试应用

  适用于: ICC/IF, Flow Cytmore details
  不适用于: IHC-Fr or IHC-P

• 种属反应性

  与反应: Human
  不与反应: Mouse

• 免疫原

  Full length native protein (purified). Extra cellular domain of the human EGFR.
  Database link: P00533

• 阳性对照

• Metastasis lung carcinoma Adenocarcinoma rectum Epithelium tabular L carcinoma

• 常规说明

  Sufficient for 100-200 tests

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at +4°C. Avoid freeze / thaw cycle.

• 存储溶液

  pH: 7.30
  Preservative: 0.1% Sodium azide
  Constituents: 1% BSA, 98.9% PBS

• 纯度

  Protein G purified

• 克隆

  单克隆

• 克隆编号

  2E9

• 骨髓瘤

  unknown

• 同种型

  IgG1

• 轻链类型

  unknown

The Abpromise guarantee

Abpromise™承诺保证使用ab8465于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用说明

Is unsuitable for IHC-Fr or IHC-P.

• 数据库链接

• Entrez Gene: 1956 Human

• Omim: 131550 Human

• SwissProt: P00533 Human

• Unigene: 488293 Human

• Unigene: 605083 Human

• 别名

• EGF R antibody

• EGFR antibody

• EGFR_HUMAN antibody

• Epidermal growth factor receptor (avian erythroblastic leukemia viral (v erb b) oncogene homolog) antibody

• Epidermal growth factor receptor (erythroblastic leukemia viral (v erb b) oncogene homolog avian) antibody

• Epidermal growth factor receptor antibody

• erb-b2 receptor tyrosine kinase 1 antibody

• ERBB antibody

• ERBB1 antibody

• Errp antibody

• HER1 antibody

• mENA antibody

• NISBD2 antibody

• Oncogen ERBB antibody

• PIG61 antibody

• Proto-oncogene c-ErbB-1 antibody

• Receptor tyrosine protein kinase ErbB 1 antibody

• Receptor tyrosine-protein kinase ErbB-1 antibody

• SA7 antibody

• Species antigen 7 antibody

• Urogastrone antibody

• v-erb-b Avian erythroblastic leukemia viral oncogen homolog antibody

• wa2 antibody

• Wa5 antibody

• Avian erythroblastic leukemia viral (v erb b) oncogene homolog antibody

• Cell growth inhibiting protein 40 antibody

• Cell proliferation inducing protein 61 antibody

• 

  Flow Cytometry - Anti-EGFR antibody [2E9] (ab8465)

  Overlay histogram showing A431 cells stained with ab8465 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab8465, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in A431 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

• 

  Immunocytochemistry/ Immunofluorescence - Anti-EGFR antibody [2E9] (ab8465)

  ICC/IF image of ab8465 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab8465, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.