抗乙型肝炎病毒Core Antigen抗体[C1]
参阅全部 Hepatitis B Virus Core Antigen 一抗
小鼠单克隆抗体[C1] to乙型肝炎病毒Core Antigen
Mouse
This antibody reacts with HBV Core Antigen (Major antigenic determinant, c1). Ab8637 should recognize both the precoreprotein and core protein. It will not recognize the precorprotein under native conditions, because this protein can not self assemble into particles.
Ab8637 was raised against serotype ayw but will work with all other genotypes.
适用于: ELISA, IP, WB, ICC/IFmore details
与反应: Hepatitis B virus
Tissue, cells or virus corresponding to Hepatitis B virus Hepatitis B Virus Core Antigen. Purified Hepatitis B Core Antigen
Around aa positions 70-80, major, or "c1" determinant
This product was raised against native assembled HBV core (HBV capsid, or particulated core).
For denaturing western blots and denaturing Immunofluorescence we would recommend one of the following:
Anti-Hepatitis B Virus Core Antigen antibody [10E11] (ab8639)
Anti-Hepatitis B Virus Core Antigen antibody [14E11] (ab8638)
These products are raised to the same, but denatured, HBcAg protein sequence thus have more robust activity.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.4
Constituents: 0.1% BSA, 99% PBS
Tissue culture supernatant
单克隆
C1
Sp2/0
IgG2a
kappa
Abpromise™承诺保证使用ab8637于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ELISA | Use at an assay dependent concentration. | |
| IP | Use at an assay dependent concentration. | |
| WB | 1/1000. We recommend boiling (100°C) the sample for 10 minutes immeditaley before loading and using a 2x or 4x Laemmli sample buffer (contains SDS). A 2X concentrated Laemmli buffer is: 4% SDS, 10% beta-mercaeptoethanol, 20% glycerol, 0.1M Tris pH 6.8, and 0.005% of bromophenol blue. Read More | |
| ICC/IF | (1) | Use at an assay dependent concentration. |
Entrez Gene: 2828293 Hepatitis B virus
Entrez Gene: 944568 Hepatitis B virus
SwissProt: P03146 Hepatitis B virus
SwissProt: P03147 Hepatitis B virus
SwissProt: P03148 Hepatitis B virus
SwissProt: P0C573 Hepatitis B virus
SwissProt: P69706 Hepatitis B virus
core antibody
Core antigen antibody
Core protein antibody
HBc antibody
HBcAg antibody
HBVgp4 antibody
Hepatitis B core antigen antibody
Hepatitis B Virus core antigen antibody
p21.5 antibody
precore/core protein antibody
C antibody
Capsid protein antibody
Core and e antigen antibody
![Western blot - Anti-Hepatitis B Virus Core Antigen antibody [C1] (ab8637)](https://www.abcam.cn/ps/products/8/ab8637/Images/ab8637_1.jpg)
Western blot - Anti-Hepatitis B Virus Core Antigen antibody [C1] (ab8637)
Immunoblotting analysis of the recombinant HBcAg antigen using ab8637. E.coli lysates were separated using 12% SDS-PAAG electrophoresis, imunoblotted and developed with the Amersham ECL Detection Kit. Samples: 1. Negative control; 2. HBcAg (full length, 1-183)
