Anti-Angiopoietin 2/ANG2抗体
参阅全部 Angiopoietin 2/ANG2 一抗
兔多克隆抗体to Angiopoietin 2/ANG2
Rabbit
Angiopoietin 2 Antibody antiserum is directed against mouse angiopoietin-2 and shows no reactivity with angiopoietin-1 from mouse-derived proteins. This reagent cross-reacts with human angiopoietin-2. Partially cross-reactivity is noted with human angiopoietin-1.
适用于: IHC-P, ICC/IF, WBmore details
与反应: Mouse, Human
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Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
pH: 7.20
Preservative: 0.1% Sodium azide
Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution, 98.5% Whole serum
Whole antiserum
Angiopoietin 2 Antibody antiserum is directed against mouse angiopoietin-2 and shows no reactivity with angiopoietin-1 from mouse-derived proteins. This product was prepared from monospecific antiserum by a delipidation and defibrination.
多克隆
IgG
Abpromise™承诺保证使用ab8452于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | (1) | 1/200 - 1/500. |
| ICC/IF | (1) | Use at an assay dependent concentration. |
| WB | (2) | 1/500 - 1/2000. There is reaction with serum in the cell supernatants, which results in strong background and makes it difficult to see the angiopoietins in cell supernatants. However when precipitated (using soluble Tie2) the signals are very good and strong. Read More |
Entrez Gene: 285 Human
Entrez Gene: 11601 Mouse
Omim: 601922 Human
SwissProt: O15123 Human
SwissProt: O35608 Mouse
Unigene: 583870 Human
Unigene: 439874 Mouse
ANG-2 antibody
ANG2 antibody
Angiopoietin 2a antibody
Angiopoietin 2B antibody
Angiopoietin-2 antibody
Angiopoietin2 antibody
ANGP2_HUMAN antibody
ANGPT 2 antibody
Angpt2 antibody
Tie2 ligand antibody
AGPT 2 antibody
Agpt2 antibody
ANG 2 antibody
Western blot - Anti-Angiopoietin 2/ANG2 antibody (ab8452)This image is courtesy of Marion Scharpfenecker 2002
Supernatants of mouse angiopoietin-expressing endothelial cells. Soluble Tie 2 was used to precipitate the angiopoietins to reduce background.
Lane 1 - mock
Lane 2 - mouse angiopoietin-2 (clone 2-9) expressing cells
Lane 3 - mouse angiopoietin-1 (clone 1-15) expressing cells
Lane 4 - mouse angiopoietin-1 (clone 1-8) expressing cells
Lane 5 - wt
Immunocytochemistry/ Immunofluorescence - Anti-Angiopoietin 2/ANG2 antibody (ab8452)
ICC/IF image of ab8452 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab8452, 1/200 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
