Anti-HIF-1 alpha antibody [ESEE122]

• 产品名称

  Anti-HIF-1 alpha抗体[ESEE122]
  参阅全部 HIF-1 alpha 一抗

• 描述

  小鼠单克隆抗体[ESEE122] to HIF-1 alpha

• 宿主

  Mouse

• 经测试应用

  适用于: ICC/IF, IHC-Pmore details
  不适用于: WB

• 种属反应性

  与反应: Human
  

• 免疫原

  Recombinant fragment corresponding to Human HIF-1 alpha aa 300-550.

• 阳性对照

• IHC-P: Human colon tissue. ICC: HeLa BrdU cells.

• 常规说明

  Under normoxic conditions HIF-1 alpha has a short half-life. It is largely undetectable in cells or tissues grown under normoxic conditions. It is stabilized only at O₂ concentrations below 5% and upon stabilization under hypoxic conditions HIF-1 translocates to the nucleus. Therefore we recommend western blots using nuclear extracts and running Hypoxia treated samples as positive control (ab180880). Hypoxia can be induced with treatment using certain agents e.g. CoCl₂ or DFO, etc. so proper sample preparation is critical.

  
  

  This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.

• 存储溶液

  pH: 7.40
  Preservative: 0.02% Sodium azide
  Constituents: PBS, 6.97% L-Arginine

• 浓度

• 10 µg 浓度为 1 mg/ml

• 100 µg 浓度为 1 mg/ml

• 纯度

  Affinity purified

• 克隆

  单克隆

• 克隆编号

  ESEE122

• 骨髓瘤

  NS1

• 同种型

  IgG1

• 轻链类型

  unknown

The Abpromise guarantee

Abpromise™承诺保证使用ab8366于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用说明

Is unsuitable for WB.

• 数据库链接

• Entrez Gene: 3091 Human

• Omim: 603348 Human

• SwissProt: Q16665 Human

• Unigene: 597216 Human

• 别名

• Basic-helix-loop-helix-PAS protein MOP1 antibody

• bHLHe78 antibody

• Class E basic helix-loop-helix protein 78 antibody

• HIF 1A antibody

• HIF 1alpha antibody

• HIF-1-alpha antibody

• HIF-1alpha antibody

• HIF-alpha antibody

• HIF1 A antibody

• HIF1 Alpha antibody

• HIF1 antibody

• HIF1-alpha antibody

• HIF1A antibody

• HIF1A_HUMAN antibody

• hifla antibody

• Hypoxia inducible factor 1 alpha antibody

• Hypoxia inducible factor 1 alpha isoform I.3 antibody

• Hypoxia inducible factor 1 alpha subunit antibody

• Hypoxia inducible factor 1 alpha subunit basic helix loop helix transcription factor antibody

• Hypoxia inducible factor 1, alpha subunit (basic helix loop helix transcription factor) antibody

• Hypoxia inducible factor1alpha antibody

• Hypoxia-inducible factor 1-alpha antibody

• Member of PAS protein 1 antibody

• Member of PAS superfamily 1 antibody

• Member of the PAS Superfamily 1 antibody

• MOP 1 antibody

• MOP1 antibody

• PAS domain-containing protein 8 antibody

• PASD 8 antibody

• PASD8 antibody

• ARNT interacting protein antibody

• ARNT-interacting protein antibody

• Basic helix loop helix PAS protein MOP1 antibody

• 

  Immunocytochemistry/ Immunofluorescence - Anti-HIF-1 alpha antibody [ESEE122] (ab8366)

  ab8366 staining HIF-1 alpha in Hela cells. Untreated and BrdU treated (10µM for 24 hours) cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab8366 at 10µg/ml and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control. Cells were then incubated with ab150117, Goat polyclonal Secondary Antibody to Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (shown in green) and ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).

  Image was acquired with a confocal microscope (Leica-Microsystems TCS SP8) and a single confocal section is shown.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIF-1 alpha antibody [ESEE122] (ab8366)

  IHC image of HIF-1-alpha staining in human normal colon formalin fixed paraffin embedded tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab8366, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

   

  For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre