Anti-HIF-2-alpha antibody [ep190b]

• 产品名称

  Anti-HIF-2-alpha抗体[ep190b]
  参阅全部 HIF-2-alpha 一抗

• 描述

  小鼠单克隆抗体[ep190b] to HIF-2-alpha

• 宿主

  Mouse

• 经测试应用

  适用于: WBmore details

• 种属反应性

  与反应: Human
  预测可用于: Rat

• 免疫原

  Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

• 阳性对照

• WB: Hypoxic A549 and HeLa cell lysate.

• 常规说明

  
  

  This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

• 存储溶液

  Preservative: 0.02% Sodium azide
  Constituent: PBS

• 浓度

• 10 µg 浓度为 1 mg/ml

• 100 µg 浓度为 1 mg/ml

• 纯度

  Affinity purified

• 克隆

  单克隆

• 克隆编号

  ep190b

• 骨髓瘤

  NS1

• 同种型

  IgG1

• 轻链类型

  kappa

The Abpromise guarantee

Abpromise™承诺保证使用ab8365于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 2034 Human

• Entrez Gene: 29452 Rat

• Omim: 603349 Human

• SwissProt: Q99814 Human

• SwissProt: Q9JHS1 Rat

• Unigene: 468410 Human

• Unigene: 55138 Rat

• 别名

• Class E basic helix-loop-helix protein 73 antibody

• ECYT4 antibody

• Endothelial PAS domain containing protein 1 antibody

• Endothelial pas domain protein 1 antibody

• Endothelial PAS domain-containing protein 1 antibody

• EPAS 1 antibody

• EPAS-1 antibody

• EPAS1 antibody

• EPAS1_HUMAN antibody

• HIF 1 alpha like factor antibody

• HIF 2 alpha antibody

• HIF-1-alpha-like factor antibody

• HIF-2-alpha antibody

• HIF2-alpha antibody

• HIF2A antibody

• HLF antibody

• Hypoxia inducible factor 2 alpha antibody

• Hypoxia inducible factor 2 alpha subunit antibody

• Hypoxia-inducible factor 2-alpha antibody

• Member of PAS protein 2 antibody

• Member of pas superfamily 2 antibody

• MOP 2 antibody

• MOP2 antibody

• PAS domain-containing protein 2 antibody

• PASD2 antibody

• Basic helix loop helix PAS protein MOP2 antibody

• Basic-helix-loop-helix-PAS protein MOP2 antibody

• bHLHe73 antibody

• 

  Western blot - Anti-HIF-2-alpha antibody [ep190b] (ab8365)

  All lanes : Anti-HIF-2-alpha antibody [ep190b] (ab8365) at 1/500 dilution
  
  Lane 1 : HeLa whole cell lysate
  Lane 2 : HeLa treated with 0.5mM DFO whole cell lysate
  
  Lysates/proteins at 20 µg per lane.
  
  Secondary
  All lanes : HRP conjugated Goat Anti-Mouse IgG (H+L) at 1/10000 dilution
  
  Developed using the ECL technique.
  
  Performed under reducing conditions.
  
  Predicted band size: 100 kDa
  Observed band size: 100 kDa
  
  
  Exposure time: 20 minutes
  
  
  

  This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 55 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab8365 overnight at 4°C. Antibody binding was detected using a Goat anti-mouse antibody conjugated to HRP, and visualised using ECL development solution ab133406.

• 

  Western blot - Anti-HIF-2-alpha antibody [ep190b] (ab8365)

  HIF-2-alpha detected in hypoxic Human lysate using ab8365. Lane 1: normoxic A549 lysate control, lane 2: hypoxic A549 lysate.