Anti-Cardiac Troponin T antibody [1C11]

• 产品名称

  Anti-Cardiac Troponin T抗体[1C11]
  参阅全部 Cardiac Troponin T 一抗

• 描述

  小鼠单克隆抗体[1C11] to Cardiac Troponin T

• 宿主

  Mouse

• 经测试应用

  适用于: ICC/IF, IHC-P, Sandwich ELISAmore details

• 种属反应性

  与反应: Mouse, Rat, Dog, Human
  

• 免疫原

  Recombinant fragment corresponding to Human Cardiac Troponin T aa 150-250.
  Database link: P45379

• 阳性对照

• Natural Human Cardiac Troponin T protein (ab9937) can be used as a positive control in WB. IHC: Human heart tissue. ICC/IF: ioSkeletal Myocytes - Human iPSC-Derived Skeletal Myocytes (ab277612).

• 常规说明

  This antibody detects Troponin T in human cardiac muscle. No cross-reaction with skeletal troponin T, cTnI and TnC.

  This product was changed from ascites to tissue culture supernatant on 17th October 2017 and product received after this date will be from tissue culture supernatant.

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.

• 存储溶液

  pH: 7.40
  Preservative: 0.1% Sodium azide

• 纯度

  Protein A purified

• 纯化说明

  Purified from TCS

• Primary antibody说明

  This antibody detects Troponin T in human cardiac muscle. No cross-reaction with skeletal troponin T, cTnI and TnC.

• 克隆

  单克隆

• 克隆编号

  1C11

• 骨髓瘤

  Sp2/0

• 同种型

  IgG1

• 轻链类型

  unknown

The Abpromise guarantee

Abpromise™承诺保证使用ab8295于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

数据库链接

• Entrez Gene: 403532 Dog

• Entrez Gene: 7139 Human

• Entrez Gene: 21956 Mouse

• Entrez Gene: 24837 Rat

• Omim: 191045 Human

• SwissProt: P45379 Human

• SwissProt: P50752 Mouse

• SwissProt: P50753 Rat

• Unigene: 533613 Human

• Unigene: 247470 Mouse

• Unigene: 9965 Rat

别名

• Cardiac muscle troponin T antibody

• Cardiomyopathy dilated 1D (autosomal dominant) antibody

• Cardiomyopathy hypertrophic 2 antibody

• CMD1D antibody

• CMH2 antibody

• CMPD2 antibody

• cTnT antibody

• LVNC6 antibody

• MGC3889 antibody

• OTTHUMP00000033864 antibody

• OTTHUMP00000033865 antibody

• OTTHUMP00000033866 antibody

• OTTHUMP00000033867 antibody

• OTTHUMP00000033870 antibody

• OTTHUMP00000218095 antibody

• RCM3 antibody

• TNNT 2 antibody

• TNNT2 antibody

• TNNT2_HUMAN antibody

• TnTc antibody

• Troponin T cardiac muscle antibody

• Troponin T type 2 (cardiac) antibody

• Troponin T type 2 cardiac antibody

• Troponin T, cardiac muscle antibody

• Troponin T2 cardiac antibody

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin T antibody [1C11] (ab8295)Ye L et al. Decreased Yes-Associated Protein-1 (YAP1) Expression in Pediatric Hearts with Ventricular Septal Defects. PLoS One 10:e0139712 (2015).

  Paraffin-embedded Normal human Heart and iVSD heart tissue (were blocked using 10% FBS for 30 min) stained for Cardiac Troponin T (Red) using ab8295 at 1/200 dilution at room temperature for 2 hours. The slides were then incubated with Fluor® 555-conjugated anti-mouse (Abcam, ab150107; 1:1,000 dilution). The nuclear counterstain was DAPI (Blue).

• 

  Immunocytochemistry/ Immunofluorescence - Anti-Cardiac Troponin T antibody [1C11] (ab8295)

  Immunofluorescence staining of Cardiac Troponin T using ab8295 in ioSkeletal Myocytes - Human iPSC-Derived Skeletal Myocytes (ab277612), which were differentiated for 10 days post induction.

  
  

  The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% PBS-Tween for 5 mins and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab8295 at 5 µg/mL and ab6046, rabbit polyclonal to beta Tubulin, at 1/1000 dilution. Cells were then incubated with ab150117, Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (shown in green) and ab150088, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed at 1/1000 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

  
  

  Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown. Gamma is adjusted to 1.5 in all channels.

  
  

  The antibody ab8295 gave comparable results using MeOH fixation (100%, 5 min).

• 

  Sandwich ELISA - Anti-Cardiac Troponin T antibody [1C11] (ab8295)

  Calibration curves for sandwich cTnT fluoroimmunoassay with different animal TnTs as antigen.(dark blue) canine, (blue/grey) human, (grey) mouse, (black) rat. Monoclonal antibodies: capture, ab8295 [clone 1C11], 1 µg/well, detection ab1454 [clone 7E7], 200 ng/well. Assay time, 30 min at room temperature.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin T antibody [1C11] (ab8295)

  Ab8295 staining human normal heart. Staining is localised to the cytoplasm.
  Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.
  Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS), then incubated with primary antibody for 20 minutes, and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.