Anti-MCP1抗体
参阅全部 MCP1 一抗
兔多克隆抗体to MCP1
Rabbit
适用于: WB, Sandwich ELISAmore details
与反应: Human
Recombinant fragment corresponding to Human MCP1.
Although some customers have been successful in IHC we no longer batch test in this application. For and IHC validated antibody please see ab73680
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Lyophilized:Reconstitute with 200µl of sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Immunogen affinity purified
多克隆
IgG
unknown
Abpromise™承诺保证使用ab9669于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (3) | Use a concentration of 0.1 - 0.2 µg/ml. To detect hMCAF/MCP-1 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant MCP-1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. Read More |
| Sandwich ELISA | Use a concentration of 0.5 - 2 µg/ml. Can be paired for Sandwich ELISA with Mouse monoclonal [2.2-4H5-1A11] to MCP1 (ab9858). To detect MCP-1 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of ab9669 is required. This antigen affinity purified antibody, in conjunction with ab271250 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant MCP-1. Read More |
Entrez Gene: 6347 Human
Omim: 158105 Human
SwissProt: P13500 Human
Unigene: 303649 Human
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Sandwich ELISA - Anti-MCP1 antibody (ab9669)
Standard curve for MCP1 (Analyte: ab73866); dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal to MCP1 (ab9858) at 1µg/ml and Detector Antibody Rabbit polyclonal to MCP1 (ab9669) at 0.5µg/ml.
Western blot - Anti-MCP1 antibody (ab9669)
All lanes : Anti-MCP1 antibody (ab9669) at 0.2 µg/ml
Lane 1 : Recombinant human MCP1 protein (ab73866) at 0.01 µg
Lane 2 : Recombinant human MCP1 protein (ab73866) at 0.001 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 1 minute
Western blot - Anti-MCP1 antibody (ab9669)Image courtesy of Dr Laura Toma by Abreview.
Anti-MCP1 antibody (ab9669) at 1/4000 dilution + lysate prepared from human smooth muscle treated with 100µg/ml glycated LDL at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/20000 dilution
Developed using the ECL technique.
Observed band size: 12-14 kDawhy is the actual band size different from the predicted?
Exposure time: 1 minute
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