Normal驴Serum
参阅全部 Donkey Serum 试剂
Donkey
适用于: IHC (PFA fixed), Blocking, IHC-P, IHC-Fr, ICC/IFmore details
Normal donkey serum ab7475 is used extensively for the blocking of non-specific antibody binding in tissue and cell staining, and in other applications of antibodies.
The donkey serum blocks the binding of Fc receptors in the sample to the primary and secondary antibodies used in the experiment, and also blocks non-specific binding of the antibodies to the sample.
Typically the serum used for blocking is from a different species than the species in which the primary antibody was raised. Often the blocking serum is from the species in which the secondary antibody was raised.
Serum can be used directly for blocking, or as a constituent of a blocking buffer.
Strain: Mixed breed and sex.
Raised in: Donkey
Purity: Whole antiserum
Liquid
Shipped on Dry Ice. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Constituent: Whole serum
Abpromise™承诺保证使用ab7475于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC (PFA fixed) | Use at an assay dependent dilution. | |
| Blocking | Use at an assay dependent concentration. | |
| IHC-P | Use at an assay dependent concentration. | |
| IHC-Fr | Use at an assay dependent concentration. | |
| ICC/IF | Use at an assay dependent dilution. PubMed: 17597760Use at 10% dilution. |
Immunohistochemistry (Frozen sections) - Normal Donkey Serum (ab7475)Paul, Rajib et al., PloS one vol. 12,2 e0171285., Fig 5, doi:10.1371/journal.pone.0171285
The sections were rinsed three times with 0.1 M Tris-buffered saline (TBS, 0.1 M; pH 7.4), incubated in 3% H2O2 in TBS, permeabilized with 0.3% Triton X-100, and blocked with 10% donkey serum containing 0.3% Triton X-100. The sections were incubated overnight with primary antibody for TH (1:700) in TBS, containing 2% donkey serum (ab7475) at 4°C and then incubated with HRP-conjugated secondary antibody (1:1000) in TBS for 1 h at room temperature.
Immunohistochemistry (PFA perfusion fixed frozen sections) - Normal Donkey Serum (ab7475)Krzy?anowska, Weronika et al., PloS one?vol. 12,10 e0186243., Fig 6, doi:10.1371/journal.pone.0186243
Anti-xCT rabbit (ab37185 Abcam) dilution: 1:200 Donkey anti-rabbit TR (ab6800 Abcam) dilution: 1:1000 Donkey normal serum (ab7475 Abcam) dilution: 10%
