Anti-Laminin 1+2 antibody

• 产品名称

  Anti-Laminin 1+2抗体

• 描述

  兔多克隆抗体to Laminin 1+2

• 宿主

  Rabbit

• 特异性

  This antibody is pan-specific and reacts well with all laminin isoforms tested: laminin-1 (alpha-1, beta-1, and gamma-1) and laminin-2 (alpha-2, beta-1, and gamma-1).

• 经测试应用

  适用于: IHC-P, WB, ICC/IFmore details

• 种属反应性

  与反应: Mouse, Rat, Human
  

• 免疫原

  Full length native protein (purified) (Mouse EHS tumour).

• 常规说明

  
  

  Laminin antibodies are widely used to label blood vessels and basement membranes.

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  Preservative: 0.05% Sodium azide

• 浓度

• 100 µl 浓度为 1 mg/ml

• 纯度

  Immunogen affinity purified

• Primary antibody说明

  Laminin antibodies are widely used to label blood vessels and basement membranes.

• 克隆

  多克隆

• 同种型

  IgG

• 
  

• The Abpromise guarantee

• Abpromise™承诺保证使用ab7463于以下的经测试应用

• “应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 应用	Ab评论	说明
  IHC-P	(1)	1/500 - 1/2000. Perform enzymatic antigen retrieval before commencing with IHC staining protocol.  Immunostaining enhanced by antigen retrieval with pepsin - apply 100ul of 4mg/ml pepsin dissolved in 0.01M HCl for 1 hour at 37C,  especially paraffin tissue. Read More
  WB	(1)	1/100 - 1/5000.  Use 5-7% of gel for electrophoresis.
  ICC/IF	(1)	1/50 - 1/200.

• 数据库链接

• Entrez Gene: 284217 Human

• Entrez Gene: 3908 Human

• Entrez Gene: 3912 Human

• Entrez Gene: 3913 Human

• Entrez Gene: 3915 Human

• Entrez Gene: 3918 Human

• Entrez Gene: 84823 Human

• Entrez Gene: 16772 Mouse

• Entrez Gene: 16773 Mouse

• Entrez Gene: 16779 Mouse

• Entrez Gene: 16782 Mouse

• Entrez Gene: 226519 Mouse

• Omim: 150240 Human

• Omim: 150290 Human

• Omim: 150292 Human

• Omim: 150320 Human

• Omim: 150325 Human

• Omim: 156225 Human

• SwissProt: P07942 Human

• SwissProt: P11047 Human

• SwissProt: P24043 Human

• SwissProt: P25391 Human

• SwissProt: Q03252 Human

• SwissProt: Q13753 Human

• SwissProt: P02468 Mouse

• SwissProt: P19137 Mouse

• SwissProt: Q8R0Y0 Mouse

别名

• LAMA antibody

• LAMA1 antibody

• LAMA2 antibody

• LAMB antibody

• LAMB1 antibody

• LAMC antibody

• LAMC1 antibody

• Laminin A chain antibody

• Laminin alpha 1 antibody

• Laminin alpha 2 antibody

• Laminin alpha 2 subunit antibody

• Laminin B1 chain antibody

• Laminin B2 chain antibody

• Laminin beta 1 antibody

• Laminin gamma 1 antibody

• Laminin M antibody

• LAMM antibody

• Merosin antibody




• 

  Immunocytochemistry/ Immunofluorescence - Anti-Laminin 1+2 antibody (ab7463)

  Ab7463 staining Laminin 1+2 in HeLa cells by ICC/IF (Immunocytochemistry/Immunofluorescence). Samples were incubated with primary antibody at 1:50 dilution. Hoechst 33258 (blue) was used as a nuclear counterstain. 

• 

  Immunocytochemistry/ Immunofluorescence - Anti-Laminin 1+2 antibody (ab7463)

  Ab7463 staining Lamin 1+2 in HeLa cells by ICC/IF (Immunocytochemistry/Immunofluorescence). Samples were incubated with primary antibody at 1:5 dilution. An Alexa Fluor® 488- conjugated Goat to rabbit IgG was used as a secondary antibody. DAPI was used as a nuclear stain and an Alexa Fluor® 568 phalloidin (red) was used to stain actin fllaments. 

• 

  Western blot - Anti-Laminin 1+2 antibody (ab7463)

  All lanes : Anti-Laminin 1+2 antibody (ab7463) at 1 µg/ml
  
  Lane 1 : Mouse EHS tumor crude extracts
  Lane 2 : Rat Heart Crude Extracts
  
  Observed band size: 200,400 kDawhy is the actual band size different from the predicted?
  
  

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin 1+2 antibody (ab7463)

  Ab7463 staining Laminin in rat spinal cord and dorsal root by immunohistochemistry using paraformaldehyde/paraffin embedded tissue. Pepsin antigen retrieval was performed on this tissue sample.

• 

  Immunocytochemistry/ Immunofluorescence - Anti-Laminin 1+2 antibody (ab7463)

  ab7463 staining Laminin 1+2 in human MDA-MB-231 by ICC/IF (Immunocytochemistry/ Immunofluorescence). The cells were paraformaldehyde fixed, permeabilized in 0.1% Triton X-100 buffer and blocked in 5% serum for 30 minutes at 25°C. The primary antibody was diluted 1/250 and incubated with sample for 1 hour at 37°C. A Alexa Fluor® 488 conjugated goat polyclonal to rabbit IgG was used undiluted, as secondary.