驴F(ab')2抗兔IgG H&L (PE)预吸附二抗
参阅全部 IgG 二抗
Donkey
Rabbit
No reaction was observed against anti-Pepsin, anti-Donkey IgG F(c) or to Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rat & Sheep Serum Proteins.
适用于: WB, IHC-P, ICC/IF, Immunomicroscopy, Flow Cytmore details
Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Human, Mouse, Rat, Sheepmore details
Rabbit IgG whole molecule
PE. Ex: 488nm, Em: 575nm
Liquid
Shipped at 4°C. Store at +4°C.
Preservative: 0.01% Sodium azide
Constituents: 1% BSA, 0.424% Potassium phosphate solution, 0.88% Sodium chloride
Immunogen affinity purified
F(ab')2 Anti-Rabbit IgG (H&L) Phycoerythrin Conjugated Antibody Pre-adsorbed was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
R-Phycoerythrin (R-PE) Molecular Weight 240,000 daltons) Absorption Wavelength: 488 nm Emission Wavelength: 575 nm OD566nm/OD280nm: 2.8
多克隆
IgG
Can be used in immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Abpromise™承诺保证使用ab7007于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | 1/1000. Predicted molecular weight: 36 kDa. | |
| IHC-P | 1/200. | |
| ICC/IF | 1/100 - 1/250. | |
| Immunomicroscopy | Use at an assay dependent concentration. | |
| Flow Cyt | 1/100 - 1/250. The maximum amount of reagent required to stain 1 x 106 cells in flow cytometry is approximately 1.0 ug of antibody conjugate. |
Flow Cytometry - Donkey F(ab')2 Anti-Rabbit IgG H&L (PE) preadsorbed (ab7007)
ab7007 was used with the primary antibody ab18203 in Flow Cyt. See Abreview on ab18203.
Immunocytochemistry/ Immunofluorescence - Donkey F(ab')2 Anti-Rabbit IgG H&L (PE) preadsorbed (ab7007)
ab7007 was used at dilution 1/250 with the primary antibody ab24182 in ICC/IF. See the review on ab24182.
