小鼠brain组织裂解物- total蛋白(0 days)
参阅全部 Brain 细胞裂解液
Source: Outbred NIH-Swiss Albino mice
The mouse brain tissue was collected from zero (0) day-old mice. The lysate was prepared by homogenization in modified RIPA buffer (50 mM Tris -HCl, pH 7.4, 1% Triton X-100, 0.2% sodium deoxycholate, 0.2% sodium dodecylsulfate (SDS), 1 mM sodium ethylenediaminetetraacetate, 1 mM phenylmethylsulfonyl flouride, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The tissue lysate was boiled for 5 min in 1 x SDS sample buffer (0.045 M Tris-HCl pH 6.8, 10% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue), containing 0.05 M DTT.
适用于: WBmore details
Yes
Liquid
Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
pH: 7.20
Constituent: 100% SDS Sample Buffer
浓度
100 µg 浓度为 2 mg/ml
Abpromise™承诺保证使用ab7188于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | Use at an assay dependent dilution. Ready to load on SDS-PAGE for Western blotting, 10-20 µg per lane is recommended for mini gel. |
Western blot - Mouse brain tissue lysate - total protein (0 days) (ab7188)
Anti-Doublecortin antibody (ab18723) at 1 µg/ml + Mouse brain tissue lysate - total protein (0 days) (ab7188) at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Observed band size: 49 kDawhy is the actual band size different from the predicted?
抱歉，暂无浏览记录
