山羊抗鸡IgY H&L (HRP)
参阅全部 IgY 二抗
Goat
Chicken
适用于: ICC/IF, Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WBmore details
chicken IgY whole molecule
HRP
Liquid
Shipped at 4°C. Store at +4°C.
Preservative: 0.01% Gentamicin sulphate
Constituents: 1% BSA, 0.424% Potassium phosphate solution, 0.88% Sodium chloride
Affinity purified
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Chicken IgG coupled to agarose beads.
Horseradish Peroxidase (HRP)
多克隆
IgG
Abpromise™承诺保证使用ab6877于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | 1/1000 - 1/10000. | |
| Dot blot | Use at an assay dependent concentration. | |
| ELISA | 1/50000. | |
| IHC-P | Use at an assay dependent concentration. | |
| IHC-Fr | Use at an assay dependent concentration. | |
| Immunomicroscopy | Use at an assay dependent concentration. | |
| WB | (1) | 1/1000 - 1/5000. |
Western blot - Goat Anti-Chicken IgY H&L (HRP) (ab6877)Lichtenfels et al PLoS One. 2012;7(7):e41345. doi: 10.1371/journal.pone.0041345. Epub 2012 Jul 20. Fig 4. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/
Representative Western blot analysis for the selected panel of differentially expressed targets in the various T cell subsets.
Western blot analyses were performed by serially probing sets of 3 membranes per donor. The expression levels of GDIR1, GSTO1, LIMS1 and PROF1 were analyzed blot 1 (upper panel), GDIR2, PRDX2 and THIO on blot 2 (middle panel) and SODM on blot 3 (lower level) of the respective membrane set.
The relative expression levels were defined by densitometric quantification normalized to the corresponding beta-actin signals, co-detected on each membrane.
Aliquots of 15 µg total lysate per sample/lane were loaded onto Tris-Tricine gels (16%T/2.5%C) and subsequently separated. Lysates generated form untreated CD45RA+ and CD45RO+ T cells were throughout the set of membranes loaded onto the lanes 1 and 3 respectively, whereas lysates generated from the corresponding T cell subsets exposure for 3 h to 5 µM H2O2 were loaded onto the lanes 2 and 4 as indicated.
The protein entry names of the targets (left) and their corresponding molecular weight (right) are listed.
Western blot - Goat Anti-Chicken IgY H&L (HRP) (ab6877)
ab6877 was used at dilution 1/1000 with the primary antibody ab25912 in WB. See the review on ab25912.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Chicken IgY H&L (HRP) (ab6877)
IHC image of beta Actin staining in human normal colon formalin fixed paraffin embedded tissue section*.
The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH 6) for 30 mins. The section was incubated with ab13822, 10 µg/ml overnight at +4°C. An HRP-conjugated secondary (ab6877, 1/500 dilution) was used for 1 hr at room temperature. The section was counterstained with hematoxylin and mounted with DPX.
The inset negative control image is taken from an identical assay without primary antibody.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Chicken IgY H&L (HRP) (ab6877)
ab6877 was used at dilution 1/200 with the primary antibody ab17517 in IHC-P. See the review on ab17517.
