兔F(ab')2抗小鼠IgG - H&L (PE),预吸附二抗
参阅全部 IgG 二抗
Rabbit
Mouse
No reaction was observed against anti-Pepsin, anti-Rabbit IgG F(c) or Human Serum Proteins.
适用于: Dot blot, ICC/IF, Immunomicroscopy, Flow Cyt, IHC-P, IHC-Frmore details
Humanmore details
Full length native Mouse IgG (purified).
PE. Ex: 488nm, Em: 575nm
Liquid
Shipped at 4°C. Store at +4°C.
Preservative: 0.01% Sodium azide
Constituents: 1% BSA, 0.424% Potassium phosphate solution, 0.88% Sodium chloride
浓度
500 µg 浓度为 0.5 mg/ml
Immunogen affinity purified
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
R-Phycoerythrin (R-PE) Molecular Weight 240,000 daltons) Absorption Wavelength: 488 nm Emission Wavelength: 575 nm OD566nm/OD280nm: 2.8
多克隆
Can be used in immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Abpromise™承诺保证使用ab7000于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
Dot blot | Use at an assay dependent concentration. | |
ICC/IF | 1/100 - 1/250. | |
Immunomicroscopy | 1/50 - 1/200. | |
Flow Cyt | 1/100 - 1/250. Use 1 x 106 cells in flow cytometry is approximately 1.0 mg of antibody conjugate. | |
IHC-P | 1/50 - 1/200. | |
IHC-Fr | 1/50 - 1/200. |