驴抗兔IgG H&L (HRP)
参阅全部 IgG 二抗
Donkey
Rabbit
适用于: WB, ELISA, Immunomicroscopy, Dot blot, ICC/IF, IHC-P, IHC-Frmore details
Rabbit IgG, whole molecule
HRP
Liquid
Shipped at 4°C. Store at +4°C.
Preservative: 0.01% Gentamicin sulphate
Constituents: 1% BSA, 0.424% Potassium phosphate solution, 0.88% Sodium chloride
浓度
1 mg 浓度为 2 mg/ml
Affinity purified
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads.
多克隆
IgG
Abpromise™承诺保证使用ab6802于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | 1/1000 - 1/3000. | |
| ELISA | 1/160000. | |
| Immunomicroscopy | Use at an assay dependent concentration. | |
| Dot blot | Use at an assay dependent concentration. | |
| ICC/IF | 1/500 - 1/2000. | |
| IHC-P | 1/500 - 1/2000. | |
| IHC-Fr | 1/500 - 1/2000. |
Western blot - Donkey Anti-Rabbit IgG H&L (HRP) (ab6802)Samson et al PLoS Genet. 2014 Oct 9;10(10):e1004588. doi: 10.1371/journal.pgen.1004588. eCollection 2014 Oct. Fig 1. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/
Canonical histones are retained in C. elegans sperm.
A–E) Western blot analysis of sperm and embryo lysates loaded with equivalent amounts of DNA.
For full details please see paper.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Donkey Anti-Rabbit IgG H&L (HRP) (ab6802)
IHC image of beta actin staining in human colon formalin fixed paraffin embedded tissue section*.
The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH 6) for 30 mins. The section was incubated with ab8227, 5 µg/ml overnight at +4°C. An HRP-conjugated secondary (ab6802, 1/2,000 dilution) was used for 1 hr at room temperature. The section was counterstained with hematoxylin and mounted with DPX.
The inset negative control image is secondary-only at 1/500 dilution.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
Western blot - Donkey Anti-Rabbit IgG H&L (HRP) (ab6802)This image is courtesy of an anonymous Abreview.
Western blot - Donkey Anti-Rabbit IgG H&L (HRP) (ab6802)This image is courtesy of an Abreview submitted by Min young Kim
Western blot - Donkey Anti-Rabbit IgG H&L (HRP) (ab6802)
Western blot - Donkey Anti-Rabbit IgG H&L (HRP) (ab6802)
Lane M: 3 µl Molecular ladder.
Lane 1: Rabbit IgG whole molecule.
Lane 2: Rabbit IgG F(ab) fragment.
Lane 3: Rabbit IgG F(c) fragment.
Lane 4: Rabbit IgM whole molecule.
Lane 5: Normal rabbit serum.
All samples were reduced. Load: 50 ng per lane. Blocked for 30 min at RT.
ab6802 used at 1:5,000 for 60 min at RT. Secondary antibody: None. Predicted/Obsevered Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM. Rabbit F(c) migrates slightly higher.
