Goat Anti-Mouse IgG H&L (Biotin)

• 产品名称

  山羊抗小鼠IgG H&L (Biotin)
  参阅全部 IgG 二抗

• 宿主

  Goat

• 靶标种属

  Mouse

• 经测试应用

  适用于: ICC/IF, ICC, Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WBmore details

• 免疫原

  mouse IgG whole molecule

• 偶联物

  Biotin

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  Preservative: 0.01% Sodium azide
  Constituents: 1% BSA, 0.424% Potassium phosphate solution, 0.88% Sodium chloride

• 浓度

• 1 mg 浓度为 2 mg/ml

• 纯度

  Affinity purified

• 纯化说明

  This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose.

• 克隆

  多克隆

• 同种型

  IgG

• 常规说明

  The conjugate is expected to have between 5 and 10 moles of biotin per mole of IgG.

The Abpromise guarantee

Abpromise™承诺保证使用ab6788于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Mouse IgG H&L (Biotin) (ab6788)

  IHC image of Histone H1 staining in a section of formalin-fixed paraffin-embedded human normal colon*.

  The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH 6) for 30 mins. The section was then incubated with ab11080, 1/1,000 dilution, for 15 mins at room temperature. A goat anti-mouse biotinylated secondary antibody (ab6788, 1/1000 dilution), was used to detect the primary, and visualized using an HRP conjugated ABC system. Streptavidin HRP was used, ab7403 at a 1/10,000 dilution. DAB was used as the chromogen (ab103723), diluted 1/100 and incubated for 10 min at room temperature. The section was then counterstained with hematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.

  For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Mouse IgG H&L (Biotin) (ab6788)

  ab6788 was used at dilution 1/500 with the primary antibody ab2866 in IHC-P. See the review on ab2866.

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  ELISA - Goat Anti-Mouse IgG H&L (Biotin) (ab6788)

  ELISA - Goat Anti-Mouse IgG H&L (Biotin) (ab6788)

  ELISA results of purified ab6788 tested against purified Mouse IgG. Each well was coated in duplicate with 1.0 µg of Mouse IgG. The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log 10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC₅₀ is defined as the titer of the antibody. Assay performed using 3% fish gelatin as blocking buffer, Streptavidin Peroxidase Conjugated and TMB substrate.