兔抗大鼠IgG H&L (HRP)
参阅全部 IgG 二抗
Rabbit
Rat
适用于: IHC-P, WB, ELISA, IHC-Frmore details
Rat IgG whole molecule
HRP
Liquid
Shipped at 4°C. Store at +4°C.
Preservative: 0.01% Gentamicin sulphate
Constituents: 1% BSA, 0.424% Potassium phosphate solution, 0.88% Sodium chloride
浓度
批次浓度范围 1 mg 浓度为 1.5 - 2 mg/ml
Affinity purified
Secondary Antibody Conjugated was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Horseradish Peroxidase (HRP)
多克隆
IgG
Abpromise™承诺保证使用ab6734于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | Use at an assay dependent concentration. | |
| WB | (1) | 1/1000 - 1/5000. |
| ELISA | 1/170000. | |
| IHC-Fr | Use at an assay dependent concentration. |
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit Anti-Rat IgG H&L (HRP) (ab6734)This image is courtesy of an anonymous Abreview.
ab25377 staining Ly6g in mouse placenta tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 5% BSA for 30 minutes at 25°C. Samples were incubated with primary antibody (1/100 in PBS/1% BSA) for 12 hours at 4°C. A HRP-conjugated rabbit anti-rat IgG H&L (ab6734) (1/200) was used as the secondary antibody.
Western blot - Rabbit Anti-Rat IgG H&L (HRP) (ab6734)
ab6734 was used at dilution 1/5000 with the primary antibody ab33731 in WB. See the review on ab33731.
Immunohistochemistry (Frozen sections) - Rabbit Anti-Rat IgG H&L (HRP) (ab6734)This image is courtesy of an anonymous Abreview.
ab6326 staining BrdU in mouse small intestine tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with acetone and blocked with protein blocking solution for 1 hour at room temperature. Samples were incubated with primary antibody (1/100) in Ab diluent for 1 hour. A HRP-conjugated rabbit anti-rat IgG H&L (ab6734) (1/500) was used as the secondary antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit Anti-Rat IgG H&L (HRP) (ab6734)
IHC image of alpha tubulin staining in human colon formalin fixed paraffin embedded tissue section*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was incubated with ab64332, 5µg/ml overnight at +4°C. An HRP-conjugated secondary (ab6734, 1/500 dilution) was used for 1hr at room temperature. The section was counterstained with haematoxylin and mounted with DPX.
The inset negative control image is taken from an identical assay without primary antibody.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
