Anti-TNF alpha抗体
参阅全部 TNF alpha 一抗
兔多克隆抗体to TNF alpha
Rabbit
The immunogen used to raise this antibody shares 78% homology with mouse and rat TNF alpha protein. Some customers have successfully used ab6671 with mouse and rat samples, however we do not batch test in these species and therefore we cannot guarantee this product will consistently work with mouse and rat. We would recommend ab205587 or ab1793 as an alternative product for use with these species.
The antibody does not recognize human TNF beta (lymphotoxin).
适用于: ELISA, IHC-P, ICC/IF, WB, Neutralisingmore details
与反应: Human, Cynomolgus monkey, Recombinant fragment
预测可用于: Mouse, Rat, Dog, Pig, Monkey, Non human primates
Recombinant full length protein corresponding to Human TNF alpha.
Database link: P01375
WB: Recombinant TNF-alpha protein.
Endotoxin content by LAL is <10 pg/ml.
Abcam recommended secondaries - Goat Anti-Rabbit HRP (ab205718) and Goat Anti-Rabbit Alexa Fluor® 488 (ab150077). Or search our wide range of secondary antibodies for use with your experiment.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.2
Constituents: 0.424% Potassium phosphate solution, 0.88% Sodium chloride
浓度
200 µg 浓度为 1 mg/ml
This antibody is primarily directed against mature 17,000 MW human TNF alpha and is useful in determining its presence in various assays. In general, this antibody also detects primate TNF alpha in the same formats using similar dilutions.
多克隆
IgG
Abpromise™承诺保证使用ab6671于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ELISA | 1/1000 - 1/5000. | |
| IHC-P | (19) | 1/100 - 1/500. |
| ICC/IF | (1) | Use at an assay dependent concentration. PubMed: 19458984 |
| WB | (10) | 1/500 - 1/2000. Predicted molecular weight: 25.6 kDa. Membrane Blocking is recommended with BSA not Milk for this product. Suitable for use as a positive control for Western blot against recombinant TNFalpha produced in E.Coli. Read More |
| Neutralising | Use at an assay dependent concentration. | |
| IF | Use at an assay dependent concentration. |
Entrez Gene: 7124 Human
Entrez Gene: 21926 Mouse
Omim: 191160 Human
SwissProt: P79337 Cynomolgus monkey
SwissProt: P01375 Human
SwissProt: P06804 Mouse
Unigene: 241570 Human
Unigene: 1293 Mouse
Unigene: 2275 Rat
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Macrophage cytotoxic factor antibody
Tnf antibody
TNF superfamily member 2 antibody
TNF superfamily, member 2 antibody
TNF, macrophage derived antibody
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TNF-a antibody
TNF-alpha antibody
TNFA antibody
TNFA_HUMAN antibody
TNFSF2 antibody
Tumor necrosis factor (TNF superfamily member 2) antibody
Tumor necrosis factor alpha antibody
Tumor necrosis factor antibody
Tumor necrosis factor ligand superfamily member 2 antibody
Tumor Necrosis Factor, Membrane Form antibody
Tumor necrosis factor, soluble form antibody
Western blot - Anti-TNF alpha antibody (ab6671)
Anti-TNF alpha antibody (ab6671) at 1/1000 dilution + Recombinant TNF alpha protein
Secondary
Goat Anti-Rabbit Dylight® 649 Conjugate at 1/20000 dilution
Predicted band size: 25.6 kDa
Additional bands at: 16 kDa (possible cleavage fragment)
Western Blot analysis, labeling TNF alpha with ab6671 at 1/1000. Blocking was with 1% BSA in TBS-T incubated for 30 minutes at room temperature. The cleavage fragment is related to the soluble isoform 77 - 223.
